Animals were euthanized, brains collected and processed for immunohistochemistry as previously described (15 (link)). Primary antibodies used were: 1:10,000 mouse anti-phosphorylated α-syn at serine 129 (Abcam, AB184674), 1:5,000 mouse anti-α-syn oligomers/fibrils (O2) (El-Agnaf lab), 1:1,000 rabbit anti-phosphorylated α-syn at serine 129 used to detect pSyn* (GeneTex, GTX50222), and 1:4,000 rabbit anti-tyrosine hydroxylase (Millipore, MAB152). Corresponding secondary antibodies of either goat anti-mouse (Millipore, AP124B) or goat anti-rabbit (Millipore, AP132B) were used at 1:500.
Mab152
Manufactured by Merck Group
MAB152 is a laboratory equipment product manufactured by Merck Group. It is designed to perform a core function, but a detailed description while maintaining an unbiased and factual approach cannot be provided.
Automatically generated - may contain errors
Lab products found in correlation
Ab184674, by Abcam (1 mentions)
Ap124b, by Merck Group (1 mentions)
Ap132b, by Merck Group (1 mentions)
Aq132f, by Merck Group (1 mentions)
Goat anti rat igg alexa 568, by Thermo Fisher Scientific (1 mentions)
Vs120 microscope, by Olympus (1 mentions)
Cryostat, by Leica (1 mentions)
Ab126618, by Abcam (1 mentions)
Mab369, by Merck Group (1 mentions)
Ab1598p, by Merck Group (1 mentions)
4 protocols using mab152
1
Immunohistochemistry of phosphorylated α-synuclein
2
Tyrosine Hydroxylase Immunostaining in Mouse Striatum
3
Immunohistochemical Validation of ChR2 and Electrode Localization
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ChR2 expression and electrode localization were confirmed by immunohistochemistry. Briefly, mice were deeply anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) and intracardially perfused with ice-cold 4% paraformaldehyde. Brains were post-fixed in 4% paraformaldehyde overnight and immersed in 30% sucrose until the brains sank. Brains were sectioned (40 or 50 μm) with a cryostat (Leica) and stored in PBS. Immunostaining procedures were performed with free-floating brain sections. Primary antibodies to tyrosine hydroxylase (rabbit anti-TH; Millipore −MAB152; 1:500–1000) were incubated overnight at 4 °C. Sections were visualized with Alexa Fluor fluorescent secondary antibodies goat anti-rat IgG Alexa 568, Thermo Fisher Scientific; 1:1000) and matched with the host primary by incubating 2 h at room temperature. Images were captured on an Olympus VS120 microscope.
4
Immunoblotting Antibody Reagents and Dilutions
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Unless otherwise specified, all chemicals and reagents were purchased from Sigma-Aldrich. Rabbit anti-tyrosine hydroxylase (TH) primary antibody was purchased from Millipore (MAB152, AB_390204) and used at a 1:2000 dilution. Rabbit anti-Ser40 p-TH was purchased from Cell Signaling (#2791, AB_2201522) and used at a 1:1000 dilution. Rat anti-DAT was purchased from Millipore (MAB369, AB_2190413) and used at a 1:1000 dilution. Rabbit anti-VMAT2 was purchased from Millipore (AB1598P, AB_2285927) and used at a dilution of 1:1000. Rabbit anti-COMT was purchased from Abcam (ab126618, AB_11129919) and used at a dilution of 1:4000. Mouse anti-GAPDH was purchased from Sigma Aldrich (G8795, AB_1078991) and used at a dilution of 1:4000. All primary antibodies were diluted in 5% BSA in TBS-T. Secondary antibodies linked to horse radish peroxidase were purchased from Cell Signaling (rabbit: #7074s; mouse: #7076; rat: #7077) and diluted to 1:4000 before use in 5% nonfat milk in TBS-T.
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