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Sars cov 2 and hku 1 spike trimers

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SARS-CoV-2 and HKU-1 spike trimers are recombinant proteins produced by BPS Biosciences. These spike trimers mimic the structure of the SARS-CoV-2 and HKU-1 coronavirus spike proteins, which are critical for viral attachment and entry into host cells.

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Lab products found in correlation

Spike 2, by ACROBiosystems (2 mentions) Magnetic carboxylated beads, by Bio-Rad (2 mentions) Sars cov 2 spike 1, by Sino Biological (2 mentions) Sars cov and nl63 spike trimers, by BPS Biosciences (2 mentions) Tetanus toxoid, by Merck Group (2 mentions) Influenza hemagglutinin h1cal2009, by Sino Biological (2 mentions) Siv gp120, by Sino Biological (2 mentions) Nucleoprotein, by ACROBiosystems (1 mentions)

Close spelling variants of this product

SARS-CoV-2 spike trimers SARS-CoV-2 spike

2 protocols using sars cov 2 and hku 1 spike trimers

1

Multiplex SARS-CoV-2 Antibody Detection

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As previously described25 , a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Fig. 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were also included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B., Selva K., Kedzierski L., Ashhurst T., Haycroft E., Shoffner S., Hensen L., Boyd D., James F., Mouhtouris E., Kwong J., Chua K., Drewett G., Copaescu A., Dobson J., Rowntree L., Habel J., Allen L., Koay H.F., Neil J., Gartner M., Lee C., Andersson P., Seemann T., Sherry N., Amanat F., Krammer F., Londrigan S., Wakim L., King N., Godfrey D., Mackay L., Thomas P., Nicholson S., Arnold K., Chung A., Holmes N., Smibert O., Trubiano J., Gordon C., Nguyen T, & Kedzierska K. (2021). Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity. Research Square.
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2

SARS-CoV-2 Multiplex Serological Assay

Cited 1 time
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As previously described25 (link), a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Table 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B.Y., Selva K.J., Kedzierski L., Ashhurst T.M., Haycroft E.R., Shoffner-Beck S.K., Hensen L., Boyd D.F., James F., Mouhtouris E., Kwong J.C., Chua K.Y., Drewett G., Copaescu A., Dobson J.E., Rowntree L.C., Habel J.R., Allen L.F., Koay H.F., Neil J.A., Gartner M.J., Lee C.Y., Andersson P., Khan S.F., Blakeway L., Wisniewski J., McMahon J.H., Vine E.E., Cunningham A.L., Audsley J., Thevarajan I., Seemann T., Sherry N.L., Amanat F., Krammer F., Londrigan S.L., Wakim L.M., King N.J., Godfrey D.I., Mackay L.K., Thomas P.G., Nicholson S., Arnold K.B., Chung A.W., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Nguyen T.H, & Kedzierska K. (2022). SARS-CoV-2 infection results in immune responses in the respiratory tract and peripheral blood that suggest mechanisms of disease severity. Nature Communications, 13, 2774.
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