USP7 CRISPR/Cas9 plasmid was purchased from Santa Cruz and transfected into B16 cells. After 48 h transfection, B16 cells were cultured into 96-well plates at a concentration of 1 cell/well. Single colonies were detected by immunoblotting analysis. USP7 knockout B16 cells and control cells were defined as B16 USP7 KO and B16 WT, respectively.
Usp7 crispr cas9 plasmid
Manufactured by Santa Cruz Biotechnology
The USP7 CRISPR/Cas9 plasmid is a molecular biology tool that enables targeted gene editing. It contains the Cas9 enzyme and a guide RNA designed to recognize and bind to the USP7 gene sequence, allowing for precise modifications to this genomic region.
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Lab products found in correlation
2 protocols using usp7 crispr cas9 plasmid
1
Generation of USP7 Knockout B16 Cells
2
Generating USP7 Knockout B16 Cells
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USP7 CRISPR/Cas9 plasmid (mouse) was purchased from Santa Cruz (Santa Cruz). This plasmid was designed to disrupt gene expression by causing a double-stand break in a 5' constitutive within the USP7 (mouse) gene. The plasmid was transfected into B16 cells. After 48h transfection, B16 cells were cultured into 96 well plates at the concentration of 1 cell/well. Single colonies were picked and detected by immunoblotting analysis. USP7 knock out B16 cells and control cells were defined as B16 USP7 KO and B16 WT, respectively.
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