Tricaprin

Manufactured by Merck Group

Sourced in United States

Tricaprin is a laboratory reagent used as a source of fatty acids. It is a triglyceride composed of three capric acid (decanoic acid) molecules. Tricaprin is commonly used in various research and analytical applications requiring a stable fatty acid compound.

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Lab products found in correlation

8 protocols using tricaprin

Isolation and Characterization of Chestnut Lipase

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Korean chestnuts (Castanea crenata) were purchased from local markets. They were peeled, sealed in a vacuum plastic bag, and stored at 4 °C for subsequent use. Before use, the chestnuts were covered with a layer of wet cotton and allowed to germinate for 3 days at 18 °C.
HiTrap DEAE Sepharose Fast Flow, HiTrap Q Sepharose Fast Flow, and HiPrep Sephacryl S-100 HR were purchased from Cytiva (Uppsala, Sweden). Trizma® base (≥ 99.9%), diethyl ether (≥ 99.9%), p-nitrophenyl palmitate (p-NPP), sodium dodecyl sulfate (SDS), isooctane (IOT), tributyrin, tricaproin, tricaprin, trilaurin, tripalmitin, and triolein (≥ 99.0%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ammonium sulfate (≥ 99.5%), Triton X-100, sodium chloride (≥ 99.5%), and agar were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Boric acid, potassium chloride, and oleic acid were obtained from Duksan Pure Chemicals Co., Ltd. (Ansan, Gyeonggi-do, Korea). The Costar® 96-well microplates (clear wall, clear bottom) used for the fluorometric assay were purchased from Corning Co. (Corning, NY, USA). All other chemicals were of analytical grade and were used without further purification.

Heo J., Kwon C.W., Lee J., Park H., Yu H, & Chang P.S. (2022). A jacalin-related lectin domain-containing lipase from chestnut (Castanea crenata): Purification, characterization, and protein identification. Current Research in Food Science, 5, 2081-2093.

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Lipid Extraction and Analysis Protocol

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Chemicals were purchased as follows: purified linseed oil (Mundo dos Óleos, Brasília, Brazil); glycerin, KOH, NaCl and anhydrous Na₂SO₄ (Synth, Diadema, Brazil); NaOH and n-heptane (Vetec, Duque de Caxias, Brazil); anhydrous ethyl alcohol and H₂SO₄ (Dinâmica, Indaiatuba, Brazil); deuterated acetone from Scielab. The internal standard for CG measurements tricaprin (1,2,3-tricapropylglycerol), the external standards (monolein, diolein and triolein), and the derivates N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) were purchased from Sigma-Aldrich, (USA) with 99.9% purity.

Hobuss C.B., da Silva F.A., dos Santos M.A., de Pereira C.M., Schulz G.A, & Bianchini D. (2020). Synthesis and characterization of monoacylglycerols through glycerolysis of ethyl esters derived from linseed oil by green processes. RSC Advances, 10(4), 2327-2336.

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Synthesis and Characterization of Dicaprin Stereoisomers

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1,2-sn- and 1,3-sn-dicaprin were from Sigma. 2,3-sn-dicaprin was prepared from tricaprin (Sigma) by performing stereospecific enzymatic hydrolysis on the sn-1 ester bond [17] (link). As expected, the surface pressure-molecular area curves obtained with 1,2-sn-and 2,3-sn-dicaprin were found to be superimposable and characteristic of the 1iquid expanded state, and showed no sign of discontinuity throughout the range of surface pressures investigated. The collapse pressure of 1,2-sn- and 2,3-sn-dicaprin was 40 mN.m⁻¹, and that of 1,3-sn-dicaprin was 32 mN.m ⁻¹[18] .

Bou Ali M., Ben Ali Y., Aissa I, & Gargouri Y. (2014). Eukaryotic Expression System Pichia pastoris Affects the Lipase Catalytic Properties: A Monolayer Study. PLoS ONE, 9(8), e104221.

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Analytical Standards for Lipid Analysis

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Tocopherol standards were obtained from Calbiochem (San Diego, CA, USA). β-carotene and chlorophyll standards were procured from Fluka (Buchs, Switzerland). Sterol standards, tricaprin, glycerin, monoolein, diolein, triolein, butanetriol, and betulin were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Acros Organics (Morris Plains, NJ, USA). All reagents used in this study were purchased from Sigma-Aldrich, Merck (Darmstadt, Germany), Chem-Lab (Zedelgem, Belgium), or Fisher Scientific (Hampton, NH, USA) and were either analytical or HPLC grade.

Uitterhaegen E., Sampaio K.A., Delbeke E.I., De Greyt W., Cerny M., Evon P., Merah O., Talou T, & Stevens C.V. (2016). Characterization of French Coriander Oil as Source of Petroselinic Acid. Molecules, 21(9), 1202.

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Glyceride Standards for Analytical Methods

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Dichloromethane (GC grade; Sigma-Aldrich, Switzerland), acetonitrile (ULC/MS grade; Biosolve, France), water (LC-MS Ultra grade; Honeywell, Germany), 2-propanol (ULC/MS grade; Biosolve, France), formic acid (ULC/MS grade; Biosolve, France), ammonium formate (LC-MS Ultra grade; Fluka, Switzerland), dimyristin (DG 28:0), dipalmitolein (DG 32:2), dipalmitin (DG 32:0), diolein (DG 36:2) and distearin (DG 36:0) (all >99%; Nu-Chek Prep, Inc, USA), tricaprilin (TG 24:0), tricaprin (TG 30:0), trilaurin (TG 36:0), trimyristin (TG 42:0) and tripalmitin (48:0) (all 99.9%; Sigma-Aldrich, USA) were used as received.
Stock solutions of glycerides were prepared in dichloromethane. A 1 μM glyceride mixture in 2 : 1 : 1 isopropanol/ acetonitrile/water was used as a quality control standard. All standard solutions were stored at -24 °C before and after analysis to prevent degradation and solvent evaporation.

Frick AA ., Kummer N ., Moraleda A , & Weyermann C . (2020). Changes in latent fingermark glyceride composition as a function of sample age using UPLC-IMS-QToF-MS^E. The Analyst, 145(12).

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Enzymatic Interesterification of RBDO

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RBDO with iodine value (IV) of 60 was obtained from PT. Salim Ivomas TBK, Indonesia. Tricaprylin, molecular sieve 4 Å and triglyceride standard mixture (tricaprin, Tricaprylin, trilaurin, trimyristin, and tripalmitin) were purchased from Sigma-Aldrich, Singapore. Lipozyme TL IM was obtained from Novozyme A/S, Denmark. Hexane, chloroform, ethanol, octanol, sodium hydroxide, acetonitrile, and acetone were analytical grade and purchased from Merck, Germany.

Utama Q.D., Sitanggang A.B., Adawiyah D.R., & Hariyadi P. (2021). Lipase-catalyzed transesterification of medium-long-medium structured lipid (MLM-SL) using palm olein and tricaprylin in packed-bed reactor (PBR). Emirates Journal of Food and Agriculture.

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Enzymatic Production of Cocoa Soapstock Lipids

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The raw material used in the experiments was an olein from cocoa (Theobroma cocoa) soapstocks, obtained from OLEOFAT TRADER S.L. (Tudela -Navarra -Spain) by acid fat-splitting. Cocoa soapstocks were provided by Moner Cocoa S.A. (La Selva del Camp -Tarragona -Spain). The enzymes Lipozyme CALB and Eversa transform 2.0 were purchased from Novozymes (Bagsvaerd -Copenhagen). Glycerol (99.9%) was purchased from Labbox, NaOH, KOH and the solvents used for analysis (ethanol, n-hexane, diethyl ether, methanol and acetone) were from Scharlab. The HMDS+TMCS+Pyridine 3:1:9 (Sylon HTP) mix used to quantify MAG, DAG and TAG and the internal standards such as 1,2,4-butanetriol and tricaprin were purchased from Supelco (Sigma Chemical Co., St Louis, MO). The standards for the calibration curves in the GC analysis, 1-Monolarin, Diolein, Triolein and Glycerol, were purchased from Larodan and the 30 mg/ml FAME mix (28 components) was purchased from Restek.

Zamorano L.S., Magaña P.C., Cisneros E.G., Martinez A., & Martín L. (2020). Cocoa olein glycerolysis with lipase <em>Candida antarctica</em> in a solvent free system. Grasas y Aceites, 71(4), 383-383.

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Characterization of Lipid Compounds

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1-Stearoyl-rac-glycerol (monostearin) ( purity ≥ 99％) , glyceryl-1,3-distearate (distearin) ( purity ≥ 99％) , tetradecane (purity ≥ 99％) , and tricaprin (purity ≥ 99％) were purchased from Sigma-Aldrich (St. Louis, USA) . BSTFA (purity ＞95％) , TMCS (purity＞98％) , and the mixture of hexamethyldisilazane and trimethylchlorosilane (TMS-HT) were purchased from Tokyo Chemical Industry (Tokyo, Japan) . Pyridine (JIS special grade) was purchased from Wako Pure Chemical Industries (Osaka, Japan) .

Satou C., Goto H., Yamazaki Y., Saitou K., Matsumoto S., Takahashi O., Miyazaki Y., Ikuta K, & Yajima Y. (2017). Modified Gas Chromatographic Method to Determine Monoacylglycerol and Diacylglycerol Contents in Edible Fats and Oils. Journal of oleo science, 66(6).

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