Millipore multiscreen hv 96 well plate

The analyses of blood samples were performed at The First Affiliated Hospital of Jinzhou Medical University using LC-MS/ MS analysis. In quality control (QC) and system conditioning process, a pooled QC sample was prepared by mixing equal volumes (10 lL) of all collected samples.
All the tests were conducted at room temperature. First, a 3-mm (diameter) disc was punched from each DBS paper. The discs were placed into the Millipore MultiScreen HV 96-well plate (Millipore, Billerica, MA) for metabolite extraction and 100-lL working solution was added into each well containing a DBS disc. The plates were centrifuged at 1,500g for 2 min after 20 min of gentle shaking. While dried samples incubated at 65 nC for 20 min using 60-ML acetyl chloride/1-butanol (10:90, v/v) mixture for derivatisation. Briefly, the derivatised samples were centrifuged again and dried as described previously. Then, each dried sample was dissolved in 100-ML fresh mobile phase solution for metabolomic analysis. The direct injection MS metabolomic analysis was conducted by using an AB Sciex 4000 QTrap system (AB Sciex, Framingham, MA). System control and data collection, and data preprocessing were using Analyst v1.6.0 software (AB Sciex), ChemoView 2.0.2 (AB Sciex), respectively. All the analytes were scanned under positive mode.