Stat3

The compound GOSS (purity ≥90%) was purchased from Santa Cruz Biotechnology, Inc. Trypsin-EDTA solution 0.25% and Alamar Blue^® (a sterile buffered solution of resazurin fluorescence dye) were purchased from Sigma-Aldrich; Merck KGaA. Dimethyl sulfoxide (DMSO), penicillin/streptomycin, and Dulbecco's Phosphate Buffer Saline (DPBS) were obtained from the American Type Culture Collection. Dulbecco's Modified Eagle Medium (DMEM), heat-inactivated fetal bovine serum (FBS), and cell culture plates were purchased from VWR International (Radnor). TNF-α, Human Cytokine Antibody Array kit (cat. no. AAH-CYT-1000), Human ELISA kits for C-C Motif Ligand 2 [CCL2, also known as monocyte chemoattractant protein-1 (MCP-1), cat. no. ELH-MCP1] and Interleukin-8 (IL-8, also known as CXCL-8, cat. no. ELH-IL-8) were purchased from RayBiotech. TURBO DNA-free™ kit (cat. no. AM1907) was purchased from Life Technologies, Inc. TRIzol^® reagent was purchased from Invitrogen; Thermo Fisher Scientific. An iScript™ cDNA Synthesis kit (cat. no. 170-8891), SsoAdvanced™ Universal SYBR^® Green Supermix (cat. no. 1725271), Human PCR primers (CCL2, IKBKE, IL-8, STAT3, MAPK1, MAPK3, CCDC88A, PIK3CD, and GAPDH) were purchased from Bio-Rad Laboratories, Inc.