Cell lines (HCT116, JURKAT) were obtained from ATCC (American Type Culture Collection) situated in Virginia, USA. D-MEM (cat no. AL111), Fetal Bovine Serum (cat no. RM10432), D-PBS (cat no. TL1006) was purchased from Himedia, Camptothecin (Cat No: C9911) was procured from Sigma Alddrich, India, Propidium Iodide (cat 556463); APO-DIRECT™ Kit (cat no. 556381); FITC Rabbit Anti- Active Caspase-3 (cat no. 560901); FITC Annexin V Apoptosis Detection Kit I (cat no. 556547); MitoScreen Kit-JC-1 (cat No. 551302) were collectively purchased from BD biosciences.
Fitc rabbit anti active caspase 3
Manufactured by BD
Sourced in United States
FITC Rabbit Anti- Active Caspase-3 is a lab equipment product that detects the presence of active caspase-3 protein. Caspase-3 is a key enzyme involved in apoptosis, or programmed cell death. The FITC (Fluorescein Isothiocyanate) label allows for fluorescent detection of the active caspase-3 protein.
Automatically generated - may contain errors
Lab products found in correlation
Fitc annexin 5 apoptosis detection kit 1, by BD (2 mentions)
Apo direct kit, by BD (2 mentions)
D pbs, by HiMedia (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Jurkat, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by HiMedia (1 mentions)
Dulbecco modified eagle medium (dmem), by HiMedia (1 mentions)
Winmdi 2.8 free software, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Winmdi 2.9 free software, by BD (1 mentions)
Facs caliber, by BD (1 mentions)
Jc 1 mitoscreen kit, by BD (1 mentions)
Propidium iodide, by BD (1 mentions)
Rpmi 1640, by HiMedia (1 mentions)
5 protocols using fitc rabbit anti active caspase 3
1
Cytotoxicity Evaluation of Cell Lines
2
Quantification of Caspase-3 Activity
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The caspase activity was assessed by the FITC rabbit anti-active caspase-3 (BD Pharmingen, USA). The cells were treated with CuE of 0, 0.625, 1.25 and 2.5 μM for 24 hours. The caspase activity was detected and inspected by the flow cytometry (FACSCalibur, BD, USA). Data was analyzed using WinMDI 2.8 free software (BD, USA).
3
Assessing Caspase 3 Activity by Flow Cytometry
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The caspase activity was assessed by the FITC rabbit anti-active caspase 3 (BD Pharmingen). The cells were treated with Cu E for 24 h. Caspase 3 activition level was then measured by the flow cytometry (FACSCalibur). Data was analyzed using WinMDI 2.9 free software (BD)
4
Caspase-3 Expression in Core-Shell Treated Cells
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The cells were cultured in RPMI medium in a 6 well plate at a density of 3 x 105 cells/2 mL and incubated in a CO2 incubator at 37°C for 24h. The spent medium was then aspirated and the cells were treated with the core-shells dispersed in fresh medium and retrieved for caspase-3 expression accordingly for four time intervals (2, 6, 12, 24h). At each time interval, the medium was removed from the wells, washed with PBS and trypsinised. Harvested cells were centrifuged at 2000 rpm and supernatant was decanted. Caspase expression was estimated using FITC Rabbit Anti- Active Caspase– 3 (BD Biosciences, Catalog no.560901). Further analysis was done in flow cytometry (BD FACS Caliber).
5
Apoptosis Assay Protocol Using FBS
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Fetal bovine serum (FBS), Dulbecco's phosphate buffered saline (DPBS), cell culture mediums of F-12K, RPMI-1640 were purchased from HiMedia (Mumbai, India). Propidium iodide (PI), APO-DIRECT™ Kit, FITC Rabbit Anti-Active Caspase-3, FITC Annexin V Apoptosis Detection Kit I, MitoScreen JC-1 Kit were obtained from BD Biosciences (San Jose, CA, USA) and CPT was procured from Sigma-Aldrich (St. Louis, MO, USA).
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