Rala 610222 specific antibody

Manufactured by BD

The RalA (610222)-specific antibody is a laboratory reagent used for the detection and analysis of the RalA protein. It is a highly specific and selective antibody that binds to the RalA protein, allowing its identification and quantification in various experimental settings. The core function of this antibody is to provide researchers with a tool to study the expression, localization, and interactions of the RalA protein, which is involved in various cellular processes.

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Lab products found in correlation

Ab6672, by Abcam (2 mentions) Hdac1 h 51, by Santa Cruz Biotechnology (2 mentions) Edta free protease inhibitor tablet, by Roche (2 mentions) Sb203580, by Selleck Chemicals (2 mentions) Stat3 c 20, by Santa Cruz Biotechnology (1 mentions)

2 protocols using rala 610222 specific antibody

Signaling Pathway Protein Analysis

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All chemicals were obtained from Sigma-Aldrich unless otherwise stated. TBK1 (3013), phosphorylated TBK1 (Ser172-5483) diluted 1:500, Stat3 (9139), phosphorylated Stat3 (Tyr705-9131), p38 (9212), phosphorylated p38 (Thr180/Tyr182-9211), tubulin (2128)-specific antibodies were purchased from Cell Signaling. HDAC1 (H-51) and Stat3 (C-20)-specific antibodies were purchased from Santa Cruz. RalA (610222)-specific antibody was obtained from BD Bioscience and used at a dilution of 1:3,000. IL-6 antibody was obtained from Abcam (ab6672). All antibodies were diluted 1:1,000 unless otherwise noted. EDTA-free protease inhibitor tablet was purchased from Roche Diagnostics. For in vitro experiments InSolution SB203,580 (1 mg ml⁻¹) was purchased from Calbiochem-EMD. For in vivo experiments SB203,580 powder was purchased from Selleck Chemicals.

Reilly S.M., Ahmadian M., Zamarron B.F., Chang L., Uhm M., Poirier B., Peng X., Krause D.M., Korytnaya E., Neidert A., Liddle C., Yu R.T., Lumeng C.N., Oral E.A., Downes M., Evans R.M, & Saltiel A.R. (2015). A subcutaneous adipose tissue–liver signalling axis controls hepatic gluconeogenesis. Nature Communications, 6, 6047.

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Signaling Pathway Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were obtained from Sigma-Aldrich unless otherwise stated. TBK1 (3013), phosphorylated TBK1 (Serl72-5483) diluted 1:500, Stat3 (9139), phosphorylated Stat3 (Tyr705-9131), p38 (9212), phosphorylated p38 (Thrl80/Tyrl82-9211), tubulin (2128)-specific antibodies were purchased from Cell Signaling. HDAC1 (H-51) and Stat3 (C-20)-specific antibodies were purchased from Santa Cruz. RalA (610222)-specific antibody was obtained from BD Bioscience and used at a dilution of 1:3,000. IL-6 antibody was obtained from Abcam (ab6672). All antibodies were diluted 1:1,000 unless otherwise noted. EDTA-free protease inhibitor tablet was purchased from Roche Diagnostics. For in vitro experiments InSolution SB203,580 (1 mg ml⁻¹) was purchased from Calbiochem-EMD. For in vivo experiments SB203,580 powder was purchased from Selleck Chemicals.

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