Topolyvinylidene fluoride membranes

Manufactured by Merck Group

Sourced in United States

Topolyvinylidene fluoride (PVDF) membranes are a type of laboratory equipment used for various applications. These membranes are made of a fluoropolymer material, which provides high chemical resistance, thermal stability, and mechanical strength. The core function of PVDF membranes is to facilitate filtration, separation, and purification processes in research and industrial settings.

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Lab products found in correlation

Protein lysate, by Beyotime (1 mentions) Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions) Amersham imager 600, by GE Healthcare (1 mentions) Hrp conjugated goat anti mouse igg, by Merck Group (1 mentions) Mouse anti gapdh, by Cell Signaling Technology (1 mentions) Anti cd44 cat no ab6124, by Abcam (1 mentions) Ripa buffer, by Roche (1 mentions) Amersham imager, by Cytiva (1 mentions)

2 protocols using topolyvinylidene fluoride membranes

Protein Extraction and Western Blot Analysis

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Total proteins in each group of cells were
extracted using RIPA (radioimmunoprecipitation) protein
lysate (Beyotime, Shanghai, China). The extracted
proteins were separated using a 12% sodium
dodecyl sulphate-polyvinylidene polyacrylamide gel
electrophoresis (SDS-PAGE) and transferred on to
polyvinylidene fluoride membranes (Millipore, USA).
Subsequently, the membranes were immersed in 5%
skim milk for 2 hours. Primary antibodies were incubated
for overnight incubation at 4°C. The next day,
the membranes were incubated with horse radish peroxidase
(HRP)-labeled secondary antibody for 2 h.
Bands were exposed using electrochemiluminescence (ECL) reagent. Glyceraldehyde 3-phosphate dehydrogenase
(GAPDH) was served as the internal control.

Zhang Y., Shi S., Lin C., Che L., Li Y., Zeng Q, & Lin W. (2023). Lncrna CASC11 aggravates diabetic nephropathy via targeting FoxO1. Journal of Medical Biochemistry, 42(3), 476-483.

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Western Blot Analysis of CD44 Expression

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Cells were lysed in RIPA buffer (Roche Diagnostics GmbH) containing a complete protease inhibitor cocktail for 15 min on ice. The bicinchoninic acid assay method (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) was used to determinate protein quantity and equivalent amounts of total proteins (30 µg) were separated by 10% SDS-PAGE and transferred topolyvinylidene fluoride membranes (Millipore, Billerica, MA, America). Membranes were blocked with 5% nonfat dried milk dissolved in TBST containing 0.1% Tween 20 for 1 h at room temperature and then incubated with following specified antibodies for 1 h at room temperature: Anti-CD44 (cat no. ab6124; dilution, 1:5,000; Abcam) and mouse anti-GAPDH (cat no. 97166; dilution, 1:5,000; Cell Signaling Technology, Inc., Danvers, MA, USA), followed by HRP-conjugated goat anti-mouse IgG (cat no. A4416; dilution, 1:5,000; Sigma-Aldrich; Merck KGaA) for 1 h at room temperature. Signals were visualized using an enhanced ECL Chemiluminescence reagent (cat no. WBKLS0500; Merck KGaA) and detected using AI600 version 1.2.0 on Amersham Imager 600 (GE Healthcare, Chicago, IL, USA).

Hu B., Ma Y., Yang Y., Zhang L., Han H, & Chen J. (2018). CD44 promotes cell proliferation in non-small cell lung cancer. Oncology Letters, 15(4), 5627-5633.

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