Immunohistochemical (IHC) staining was performed using monoclonal primary antibody anti-IL-6 (ABIN6002527, antibodies-online, Germany) and anti-TNF-a (ABIN3023860, antibodies-online, Germany) to assess macrophage expression. Macrophages were counted in the periapical region. Histopathological and immunohistochemical specimens were analyzed microscopically using a light microscope Nikon Eclipse Ci-E (Nikon Corp, Japan) focusing in the periapical area of apical periodontitis. Expression of IL-6 and TNF-a of macrophages was counted from IHC-stained specimens at 400× magnification.
Eclipse ci e
The Eclipse Ci-E is a microscope designed for laboratory use. It features optical components and illumination systems to enable high-quality imaging and observation of specimens.
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16 protocols using eclipse ci e
Evaluating Inflammatory Cytokines in Apical Periodontitis
Immunohistochemical (IHC) staining was performed using monoclonal primary antibody anti-IL-6 (ABIN6002527, antibodies-online, Germany) and anti-TNF-a (ABIN3023860, antibodies-online, Germany) to assess macrophage expression. Macrophages were counted in the periapical region. Histopathological and immunohistochemical specimens were analyzed microscopically using a light microscope Nikon Eclipse Ci-E (Nikon Corp, Japan) focusing in the periapical area of apical periodontitis. Expression of IL-6 and TNF-a of macrophages was counted from IHC-stained specimens at 400× magnification.
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