2 7 dichlorofluorescein dcf diacetate h2dcfda

Myricetin (Fig. 1A) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The compound was dissolved in 100% dimethyl sulfoxide (DMSO). A 100 mmol/L stock solution of Myricetin was prepared and stored as small aliquots at −20°C until needed. We purchased MTT, DMSO, gelatin, and Horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit antibodies from Sigma-Aldrich. Growth factor-reduced Matrigel was purchased from BD Biosciences (San Jose, CA, USA). The specific antibodies against PI3K, PDK1, AKT, mTOR, and their phospho forms, anti-procaspase-3 antibody, and the AKT inhibitor LY294002 were purchased from Cell Signaling Technology (Danvers, MA, USA). The HRP-conjugated β-actin, p53, and Bax antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). 2′,7′-dichlorofluorescein (DCF) diacetate (H₂DCFDA) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA).

HMH (Fig. 1A; Sigma-Aldrich Co., St. Louis, MO, USA) was dissolved in 100% dimethyl sulfoxide (DMSO). A 50 mmol/L stock solution of HMH was prepared and stored as small aliquots at −20°C until needed. DMSO, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were purchased from Sigma-Aldrich, an apoptosis detection kit was purchased from BD Biosciences (Franklin Lakes, NJ, USA), and 2,7-dichlorofluorescein (DCF) diacetate (H₂DCFDA) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Phosphospecific anti-JNK, anti-p38, anti-ERK, anti-PI3K, anti-AKT, and anti-mTOR antibodies, anti-caspase-3, anti-caspase-9, anti-poly(ADP-ribose) polymerase (PARP), anti-Bax, anti-Bcl-2, anti-JNK, anti-p38, anti-ERK, anti-PI3K, anti-AKT, and anti-mTOR specific antibodies, the ERK inhibitor PD98059, and the AKT inhibitor LY294002 were purchased from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated b-actin, p53, p21, p-cdc2, cdc2, cyclin B1, and Rb antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).