Chemicals were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) unless otherwise noted. NMR spectra were obtained using a Bruker AMX 500 (Bruker BioSpin K.K., Yokohama, Japan) spectrometer, and residual solvents or tert-butanol were used as the internal standard (tert-butanol (deuterium oxide): 1H 1.24 ppm, 13C 30.29 ppm; methanol-d4: 1H 3.30 ppm, 13C 49.0 ppm; pyridine-d5: 1H 8.74 ppm, 13C 150.35 ppm). Mass spectra were obtained using a Waters LCT Premier Spectrometer (Waters Co., Milford, MA, USA). Specific rotations were measured using a Jasco P-2200 polarimeter (Jasco Co., Tokyo, Japan).
Lct premier spectrometer
Manufactured by Waters Corporation
Sourced in United States
The LCT Premier spectrometer is a high-performance liquid chromatography-mass spectrometry (LC-MS) system designed for analytical applications. It provides accurate mass measurements and reliable identification of compounds in complex samples.
Automatically generated - may contain errors
4 protocols using lct premier spectrometer
1
NMR and Mass Spectroscopy Characterization
2
Purification and Characterization of Lotus Plumule
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All commercially available chemicals were used without further purification.
Chemicals and enzymes were purchased from Wako Pure Chemical Industries, unless otherwise specified. Fluorescence was measured by a Synergy™ MX microplate reader (BioTek Instruments, Inc.). A Bruker AMX 500 (Bruker BioSpin K.K.) was used to obtain nuclear magnetic resonance (NMR) spectra and tert-butanol was used as an internal standard ( 1 H NMR: 1.24 ppm, 13 C NMR: 30.29 ppm). A Waters LCT Premier spectrometer (Waters Co.) was used to obtain electrospray ionisation mass spectra (ESI-MS). Lotus plumule (germ of Nelumbo nucifera Gaertn. seed) was purchased commercially from the Kampo market.
Chemicals and enzymes were purchased from Wako Pure Chemical Industries, unless otherwise specified. Fluorescence was measured by a Synergy™ MX microplate reader (BioTek Instruments, Inc.). A Bruker AMX 500 (Bruker BioSpin K.K.) was used to obtain nuclear magnetic resonance (NMR) spectra and tert-butanol was used as an internal standard ( 1 H NMR: 1.24 ppm, 13 C NMR: 30.29 ppm). A Waters LCT Premier spectrometer (Waters Co.) was used to obtain electrospray ionisation mass spectra (ESI-MS). Lotus plumule (germ of Nelumbo nucifera Gaertn. seed) was purchased commercially from the Kampo market.
3
Polymer Functionalization Reaction Protocols
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Unless otherwise stated, all polymer functionalization reactions were performed in glass vials, under ambient atmosphere. Reactions at elevated temperature were controlled using a Corning PC 420D thermostated hotplate equipped with a thermocouple probe. Room temperature reactions were performed at ca. 20 o C ambient temperature. Glacial acetic acid, AcOH was used as received from Fisher Scientific. The PHCKRM peptide was obtained from NeoBioLab and was reported 96.7% pure. Poly(S-methylmethionine sulfonium chloride), M Me , and poly(S-benzylmethionine sulfonium chloride), M Bn , were prepared as previously described. 15 Epoxides were either purchased and used as received, or synthesized (see SI). Dialysis was performed using deionized water (18.2 MΩ-cm) prepared by passing in-house deionized water through a Millipore Milli-Q Biocel A10 unit.
Regenerated cellulose dialysis tubing obtained from Spectrum Labs. ESI-MS was performed using a Waters LCT Premier spectrometer.
Regenerated cellulose dialysis tubing obtained from Spectrum Labs. ESI-MS was performed using a Waters LCT Premier spectrometer.
4
Chemical Characterization of Catecholamines
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L-epinephrine bitartrate, L-norepinephrine bitartate, and dopamine hydrochloride were purchased from Tokyo Chemical Industry (Tokyo, Japan). Other chemicals were purchased from Wako Pure Chemical Industries (Osaka, Japan) unless specified otherwise. Fluorescence was measured using a Synergy™ MX microplate reader (BioTek Instruments, Winooski, VT, USA). An AMX 500 (Bruker, Billerica, MA, USA) or JNM-EX 270 (Jeol Ltd., Tokyo, Japan) system was used to obtain nuclear magnetic resonance (NMR) spectra. Tert-butanol or residual solvent was used as an internal standard (D2O: 1 H NMR 1.24 ppm, 13 C NMR: 30.29 ppm; methanol-d4: 1 H NMR 3.30 ppm, 13 C NMR: 49.00 ppm). A LCT Premier spectrometer (Waters, Milford, MA, USA) was used to obtain electrospray ionization mass spectra (ESI MS).
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