All surgical procedures were performed stereotaxically under aseptic conditions, as described previously [31 (link), 44 (link)]. Briefly, while animals were under isoflurane anesthesia (5% induction; 2%–3% maintenance in 100% oxygen), electrodes were chronically implanted to enable recording of cortical electroencephalogram (EEG), hippocampal EEG, and nuchal electromyogram (EMG). All electrodes were secured to the skull with dental acrylic, crimped to miniconnector pins, and brought together in a plastic connector, which was secured to the skull with additional dental acrylic. Upon completion of the surgical procedure, animals were administered saline (5 cc, s.c.) to prevent dehydration. Postoperative pain was controlled with buprenorphine (0.05 mg/kg, I.M.; Cerilliant, Round Rock, TX, USA).
Buprenorphine
Manufactured by Cerilliant
Sourced in United States
Buprenorphine is a lab equipment product provided by Cerilliant. It is a reference standard used for analytical testing and research purposes. The core function of this product is to serve as a calibration and quality control material for the quantitative analysis of buprenorphine in various matrices.
Automatically generated - may contain errors
Lab products found in correlation
Naloxone, by Cerilliant (4 mentions)
Oxymorphone, by Cerilliant (3 mentions)
Hydromorphone, by Cerilliant (3 mentions)
Morphine, by Cerilliant (2 mentions)
Naltrexone, by Cerilliant (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Ammonium formate, by Merck Group (2 mentions)
Benzoylecgonine, by Cerilliant (2 mentions)
Codeine, by Cerilliant (2 mentions)
Nordiazepam, by Cerilliant (2 mentions)
Oxycodone, by Cerilliant (2 mentions)
Hydrocodone, by Cerilliant (2 mentions)
Clonazepam, by Cerilliant (2 mentions)
Lorazepam, by Cerilliant (2 mentions)
Meperidine, by Cerilliant (2 mentions)
Methadone, by Cerilliant (2 mentions)
Norbuprenorphine, by Cerilliant (2 mentions)
Oxazepam, by Cerilliant (2 mentions)
Temazepam, by Cerilliant (2 mentions)
Fentanyl, by Cerilliant (2 mentions)
7 protocols using buprenorphine
1
Chronic Implantation of Electrodes for EEG and EMG Recording
2
Opioid Receptor Binding Assay
3
Certified Reference Standards for Drugs
4
Quantitative Analysis of Opioids and Antipsychotics
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Surine DOA (synthetic urine) was purchased from Dyna-Tek Industries (Lenexa, KS). Buprenorphine, norBuprenorphine, naloxone and their respective deuterated internal standards were obtained from Cerilliant (Austin, TX). For quetiapine metabolite characterization experiments, quetiapine, 7-hydroxyquetiapine and quetiapine acid were also acquired from Cerilliant, and quetiapine sulfoxide was purchased from Toronto Research Chemicals (Toronto, Ontario, Canada). HPLC-grade acetonitrile, methanol and ammonium hydroxide (ACS grade) were obtained from Thermo Fisher (Waltham, MA). Formic acid (98%, ACS grade) was purchased from EMD Millipore (Billerica, MA), and phosphoric acid (ACS grade) was purchased from Sigma-Aldrich (St. Louis, MO). Purified water was supplied by an in-house MilliQ system (EMD Millipore). IMCSzyme recombinant β-glucuronidase and Rapid Hydrolysis Buffer (pH 7.2–7.8), used for enzymatic hydrolysis in all laboratories, were purchased from IMCS (Integrated Micro-Chromatography Systems, Irmo, SC).
5
Buprenorphine-loaded PLGA Nanoparticles
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Buprenorphine HCl (BUP) EP/USP grade was purchased from Macfarlan Smith Limited (Edinburgh, United Kingdom). Buprenorphine and Buprenorphine-D4 (BUP-D4) standards in methanol (1 mg/mL) were obtained from Cerilliant (Round Rock, TX). Different types of PLGA 50:50 (Resomer RG 502, inherent viscosity (IV) = 0.20–0.22 dL/g), Resomer RG 502 H, IV = 0.20–0.22 dL/g, Resomer 503 H, IV = 0.38 dL/g) were purchased from Evonik (Essen, Germany). Polyvinylalcohol (PVA; Mw ≈67,000; 86.7–88.7 mol % hydrolysis), tris buffer and sucrose were obtained from Sigma Aldrich (St. Louis, MO). N,N-Dimethylformamide (analytical grade), dichloromethane (HPLC grade), methanol (HPLC grade), acetonitrile (MS grade), ammonium acetate (analytical grade) and sodiumlaurylsulfate (SDS) were obtained from Roth (Karlsruhe, Germany). HEPES was purchased from PanReac Apllichem (Darmstadt, Germany), sodium chloride (NaCl) analytical grade, formic acid and acetic acid (glacial) analytical grade from Merck (Darmstadt, Germany).
6
Comprehensive Analytical Reference Standards
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Certified reference materials of amitriptyline, bupropion, citalopram, desipramine, desmethylcitalopram, duloxetine, fluoxetine, imipramine, mirtazapine, nortriptyline, paroxetine, sertraline and trazodone were purchased from LGC Standards (Teddington, London, UK); certified reference materials of clomipramine, desmethylvenlafaxine, doxepin, hydroxybupropion, norfluoxetine, norsertraline, trimipramine, venlafaxine, 7-aminoclonazepam, 7-aminoflunitrazepam, alprazolam, bromazepam, clonazepam, diazepam, flunitrazepam, lorazepam, midazolam, nitrazepam, nordiazepam, oxazepam, temazepam, zolpidem, 6-monoacetylmorphine, N-desmethyltramadol, buprenorphine, codeine, fentanyl, hydrocodone, hydromorphone, meperidine, methadone, morphine, naloxone, naltrexone, oxycodone, oxymorphone, tramadol, bupropion-d 9 , citalopram-d 6 , clonazepam-d 4 , codeine-d 3 , diazepam-d 5 , duloxetine-d 3 and morphine-d 3 from Cerilliant (Round Rock, TX, USA); methanol, acetonitrile, ammonium formate and formic acid from Merck (Darmstadt, Germany); ultrapure deionized water was purified by Milli-Q from Millipore (Billerica, MA, USA). All solvents used in the extraction procedure were HPLC grade. Quantisal™ oral fluid collection devices and elution buffer were purchased from Immunalysis (Pomona, CA, USA).
7
Quantification of Pharmaceutical Markers
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All chemicals and their corresponding isotopically-labeled analogs were obtained from various vendors. Acetaminophen, Acetaminophen-d4, amitriptyline, amitriptyline-d3, aripiprazole, aripiprazole-d8, benzoylecgonine, benzoylecgonine-d3, buprenorphine, buprenorphine-d4, caffeine, carbamazepine, carbamazepine-d10, diltiazem, diphenhydramine, diphenhydramine-d3, duloxetine, duloxetine-d3, fluoxetine, fluoxetine-d6, ketamine, ketamine-d3, methylphenidate, methylphenidate-d9, norfluoxetine, norfluoxetine-d6, promethazine, promethazine-d3, and sertraline were purchased as certified analytical standards from Cerilliant (Round Rock, TX, USA). Diamond UV water purification system was used throughout sample analysis to provide 18 MΩ water.
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