I control 1.8 sp1 microplate reader software

Manufactured by Tecan

The I-control 1.8 SP1 is a microplate reader software developed by Tecan. It provides the core functionality to operate and control Tecan's microplate readers. The software enables users to set up, run, and analyze experiments with Tecan's microplate reader hardware.

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Lab products found in correlation

Nunc f96 microwell black polystyrene plate, by Thermo Fisher Scientific (3 mentions) Infinite f200 pro, by Tecan (3 mentions) Dual luciferase assay system, by Promega (2 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

Spelling variants of this product

Microplate reader (2786 citations) I-control software (91 citations) I-control 1.7 Software (31 citations) I-control (25 citations) I-control microplate reader software (16 citations)

3 protocols using i control 1.8 sp1 microplate reader software

Dual Luciferase Assay Protocol

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For luciferase assay, cells were harvested at different timepoints as indicated in figure legends. Following lysis, cells were transferred to a Nunc F96 MicroWell Black Polystyrene Plate (137101, Thermo Scientific) and luminescence of Firefly and Renilla luciferases were measured consecutively using Dual Luciferase Assay System (Promega), infinite F200 PRO, and i-control 1.8 SP1 microplate reader software (Tecan).

Konieczny P., Mukherjee S., Stepniak-Konieczna E., Taylor K., Niewiadomska D., Piasecka A., Walczak A., Baud A., Dohno C., Nakatani K, & Sobczak K. (2021). Cyclic mismatch binding ligands interact with disease-associated CGG trinucleotide repeats in RNA and suppress their translation. Nucleic Acids Research, 49(16), 9479-9495.

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Dual Luciferase Assay for Mbnl1 UTR

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HeLa cells were grown on a Nunc F96 MicroWell Black Polystyrene Plate (137101, Thermo Scientific). Fifty nanogram of Mbnl1_e1_5΄UTR2_Luc2/Mbnl1_5΄UTR2_Luc2 plasmid were co-delivered with 150/350 ng of EGFP_MBNL1/EGFP or EGFP_MBNL1 construct with X-tremeGENE HP DNA Transfection Reagent. The cells were lysed and luminescence of firefly and Renilla luciferases was measured consecutively using Dual Luciferase Reporter Assay System (Promega), infinite F200 PRO, and i-control 1.8 SP1 microplate reader software (Tecan). Data sets obtained for Ctrl and Δ1 plasmids were normalized to the Emp plasmid data set.

Konieczny P., Stepniak-Konieczna E., Taylor K., Sznajder Ł.J, & Sobczak K. (2016). Autoregulation of MBNL1 function by exon 1 exclusion from MBNL1 transcript. Nucleic Acids Research, 45(4), 1760-1775.

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Dual Luciferase Assay Protocol

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For luciferase assay (Figures 3C, 5A,B) cells were harvested at different timepoints as indicated in figure legends. Following the lysis, the cells were transferred to a Nunc F96 MicroWell Black Polystyrene Plate (137101, Thermo Scientific) and luminescence of Firefly and Renilla luciferases were measured consecutively using Dual Luciferase Assay System (Promega), infinite F200 PRO, and i-control 1.8 SP1 microplate reader software (Tecan).

Derbis M., Konieczny P., Walczak A., Sekrecki M, & Sobczak K. (2018). Quantitative Evaluation of Toxic Polyglycine Biosynthesis and Aggregation in Cell Models Expressing Expanded CGG Repeats. Frontiers in Genetics, 9, 216.

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