The OGTT and IPITT were performed at weekly intervals following a previously reported method [53 (link)]. Briefly, mice were fasted for 16 h (overnight fasting) for the OGTT and 4 h (morning fasting) for the IPITT. Using a GLUCOCARD G+ blood glucose meter (Arkray, Kyoto, Japan), blood glucose levels were measured at 0, 15, 30, 60, and 120 min after oral glucose administration for the OGTT (1 g/kg weight) and intraperitoneal insulin injection for the IPITT (0.75 units/kg). A 35-µL blood sample was collected from the tail vein using a heparinized plastic hematocrit tube (Drummond Scientific Company, Broomall, PA, USA), and the plasma was separated by centrifugation at 2500× g for 30 min. After centrifugation, the plasma was stored at −80 °C for insulin measurement. A LBIS Insulin-Mouse-U ELISA kit (Shibayagi, Gunma, Japan) was used to measure insulin levels. The AUC and inverse AUC for blood glucose were calculated by the linear trapezoidal method for both the OGTT and the IPITT.
Glucocard g blood glucose meter
Manufactured by Arkray
Sourced in Japan
The GLUCOCARD G+ blood glucose meter is a compact and portable device designed for measuring blood glucose levels. It provides accurate and reliable results to support diabetes management.
Automatically generated - may contain errors
3 protocols using glucocard g blood glucose meter
1
Glucose and Insulin Tolerance Tests
2
Measuring Metabolic Biomarkers in Mice
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Blood levels of glucose, lipids, and insulin at the experimental endpoint were measured using appropriate equipment, reagents, and kits. The GLUCOCARD G+ blood glucose meter (Arkray) was used to measure the glucose content. The LBIS Insulin-Mouse-T ELISA kit (Shibayagi) was used to measure the insulin levels. The leptin ELISA Kit (Morinaga Institute of Biological Science, Yokohama, Japan) was used to measure leptin levels. The Triglyceride E-Test Wako (Wako Pure Chemical Industries, Osaka, Japan) was used to measure the triglyceride levels. The NEFA C-Test Wako (Wako Pure Chemical Industries) was used to measure the free fatty acid levels.
3
Glucose and Insulin Tolerance Tests
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The OGTT and IPITT were performed at weekly intervals following a previously reported method [51] (link). Briefly, mice were fasted for 16 h (overnight fasting) for the OGTT and 4 h (morning fasting) for the IPITT. Using a GLUCOCARD G+ blood glucose meter (Arkray, (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Kyoto, Japan), blood glucose levels were measured at 0, 15, 30, 60, and 120 min after oral glucose administration for the OGTT (1 g/kg body mass) and intraperitoneal insulin injection for the IPITT (0.75 units/kg). A 35-µL blood sample was collected from the tail vein using a heparinized plastic hematocrit tube (Drummond Scientific Company, Broomall, PA, USA), and the plasma was separated by centrifugation at 2,500 × g for 30 min. After centrifugation, the plasma was stored at -80°C for insulin measurement. A Rebis Insulin-Mouse-U ELISA kit (Shibayagi, Gunma, Japan) was used to measure insulin levels. The AUC and inverse AUC for blood glucose were calculated by the linear trapezoidal method for both the OGTT and the IPITT.
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