Total gpx assay kit

Manufactured by Beyotime

Sourced in China

The Total GPx Assay Kit is a laboratory tool designed to measure the total activity of glutathione peroxidase (GPx) enzymes in various biological samples. GPx enzymes play a crucial role in the antioxidant defense system by catalyzing the reduction of hydrogen peroxide and organic hydroperoxides, thereby protecting cells from oxidative damage. The kit provides a quantitative colorimetric assay to determine the total GPx activity in a sample.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using total gpx assay kit

Evaluating NAC and PAT Cytotoxicity Mechanisms

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-Acetyl-1-cysteine (NAC, purity ≥ 98%) and patulin (PAT, purity ≥ 99%) were supplied by Sigma-Aldrich (St. Louis, MO, USA). The LDH-assay kit, ROS assay kit, total SOD assay kit, annexin V–FITC apoptosis assay kit, Hoechst 33342 dyes, GSH and GSSG assay kit, GR assay kit, total GPx assay kit, ATP assay kit, mitochondrial membrane potential (MMP) assay kit, the assay kits of caspase 3, 8, and 9, and BCA protein-assay kit were obtained from Beyotime Institute of Biotechnology (Beijing, China). MitoSOX Red Mitochondrial Superoxide Indicator was purchased from Invitrogen Corporation (St. Louis, MO, USA). The CAT test kit was obtained from Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China). The Ultrapure RNA kit, Super RT cDNA kit, and UltraSYBR mixture were purchased from CWBIO (Beijing, China).

Liu J., Liu Q., Han J., Feng J., Guo T., Li Z., Min F., Jin R, & Peng X. (2021). N-Acetylcysteine Inhibits Patulin-Induced Apoptosis by Affecting ROS-Mediated Oxidative Damage Pathway. Toxins, 13(9), 595.

+ Open protocol

+ Expand

Measuring Oxidative Stress in Murine Tears and Cornea

Check if the same lab product or an alternative is used in the 5 most similar protocols

The SD or control mice were anesthetized with ketamine/xylazine. To harvest tears, 2 μL of PBS was placed on the ocular surface of each eye. Following 10 s of mixture, 2 μL of irrigated tears were harvested by pipette and immediately diluted to 98 μL with PBS. For murine corneal epithelium sample collection, the whole corneal epithelial layer was removed by using a corneal rust ring remover (Algerbrush II, USA) and was immediately transferred into 100 μL of cold PBS. After homogenizing the tissue, the supernatant was collected for further detection. H₂O₂ concentration in tear and corneal epithelium samples was measured using the Amplex Red H₂O₂/peroxidase assay kit (Invitrogen, Carlsbad, CA). Total antioxidant capacity was determined using the FRAP antioxidant assay kit (Beyotime, Shanghai, China). The GSH and GPX were detected using the total GSH assay kit (Beyotime, Shanghai, China) and total GPX assay kit (Beyotime, Shanghai, China), respectively.

Li S., Tang L., Zhou J., Anchouche S., Li D., Yang Y., Liu Z., Wu J., Hu J., Zhou Y., Yin J., Liu Z, & Li W. (2022). Sleep deprivation induces corneal epithelial progenitor cell over-expansion through disruption of redox homeostasis in the tear film. Stem Cell Reports, 17(5), 1105-1119.

+ Open protocol

+ Expand

Comprehensive Metabolic Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formic acid (HPLC grade) was purchased from Dikma Technologies Inc. (Lake Forest, CA, USA). Ultra-pure water was obtained from Hangzhou Wahaha Group Co., Ltd. (Zhejiang, China). Methanol (HPLC grade), acetonitrile (HPLC grade), chloroform (HPLC grade), isopropanol (HPLC grade), Tween, pCPA, serotonin hydrochloride, 5-hydroxyindoleacetate, kynurenine, kynurenate, 3-hydroxykynurenine, phenylalanine, tyrosine, sodium hippurate hydrate, creatinine, guanosine, hypo xanthine, 3-indolepropionic acid, citrate, oxoglutarate, succinate, xanthine, uric acid and indoxyl sulfate potassium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). T-AOC assay kit with Ferric Reducing Ability of Plasma (FRAP) method, SOD assay kit, total GPx assay Kit and lipid peroxidantion MDA assay kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). CAT assay kit and reactive oxygen species assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China).

Weng R., Shen S., Tian Y., Burton C., Xu X., Liu Y., Chang C., Bai Y, & Liu H. (2015). Metabolomics Approach Reveals Integrated Metabolic Network Associated with Serotonin Deficiency. Scientific Reports, 5, 11864.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!