Sc 36563

Manufactured by Santa Cruz Biotechnology

Sourced in Germany

Sc-36563 is a laboratory equipment product from Santa Cruz Biotechnology. It is a fundamental research tool designed to facilitate specific experimental procedures. The core function of this product is to enable researchers to conduct their investigations effectively and efficiently.

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Lab products found in correlation

4 protocols using sc 36563

Molecular Profiling of Angiogenic Signaling Pathways

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VEGF-A165 was obtained from Sigma-Aldrich (St. Louis, MO; V5765). SiRNA for FUNDC1, IP3R1, VEGFR2, serum response factor (SRF), and controls were obtained from Santa Cruz Biotechnology (sc-36563, sc-91118, sc-42475, and sc-29318; Dallas, Texas). Antibodies against the following proteins were used as the primary antibodies: CD31 (BD Pharmingen, San Jose, CA; 550274; Cell Signaling Technology, Danvers, MA;77699); β-actin and GAPDH (Santa Cruz Biotechnology, Dallas, Texas; sc-47778 and sc-137179); PCNA, Calreticulin, Cyt C, VDAC1, mitofusin-2 (MFN2), SRF, phosphorylated (p) SRF, VEGFR1, VEGFR2, pVEGFR2, and VEGFR3 (Cell Signaling Technology, Danvers, MA; 13110, 12238, 4280,4661, 9482, 5147,4261, 2893, 2478, 2479, 3408); IP3R1 (Abcam, Cambridge, MA; ab5804; ThermoFisher, Waltham, MA, PA1-901); calnexin (Abcam, Cambridge, MA; ab22595); FUNDC1 (Aviva Systems Biology, San Diego, CA; ARP53281), FUNDC1 (Novus Biologicals, LLC, Littleton CO, NBP1-81063; EMD Millipore Corporation, Temecula, CA, ABC506).

Wang C., Dai X., Wu S., Xu W., Song P, & Huang K. (2021). FUNDC1-dependent mitochondria-associated endoplasmic reticulum membranes are involved in angiogenesis and neoangiogenesis. Nature Communications, 12, 2616.

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SRF Transfection and Validation

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The siRNA plasmids that recognize human SRF (sc-36563) were purchased from Santa Cruz Biotechnology for the transient transfections. The full-length human SRF plasmids were a gift from Dr. Eric Olson (Department of Molecular Biology, UT Southwestern Medical Center at Dallas, USA). The transfections were performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions. Real-time PCR or Western blot analysis was used to assess the transfection efficiency.

He L., Lou W., Ji L., Liang W., Zhou M., Xu G., Zhao L., Huang C., Li R., Wang H., Chen X, & Sun S. (2014). Serum Response Factor Accelerates the High Glucose-Induced Epithelial-to-Mesenchymal Transition (EMT) via Snail Signaling in Human Peritoneal Mesothelial Cells. PLoS ONE, 9(10), e108593.

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Silencing SRF in HCT116 Cells

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Serum response factor (SRF) small interfering RNA (siRNA) (sc-36563, Santa Cruz) or negative control siRNA (Bioneer, Daejeon, Korea) were transfected in HCT116 cells using PepMuteTM siRNA Transfection Reagent (SignaGen).

Boonruang K., Kim I., Kwag C., Ryu J, & Baek S.J. (2023). Quercetin induces dual specificity phosphatase 5 via serum response factor. BMB Reports, 56(9), 508-513.

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Suppressing CTCF and SRF in Panc1 Cells

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To suppress expression of CTCF and SRF in Panc1 cells for luciferase assays, 1 × 10⁵ Panc1 cells per well were seeded in 12-well-plates containing 1 mL medium per well. After 24 h, medium was removed and 1 mL fresh medium was added. Then, 6 µL per well HiperFect reagent (Qiagen, Hilden, Germany) and 2.5 µL of either negative control siRNA (sc-37007, Santa Cruz) or specific siRNA against SRF (sc-36563, Santa Cruz) or CTCF (sc-35124; Santa Cruz), all in a concentration of 25 nM, were mixed with 100 µL FCS-free medium and added to cells. After 72 h, cells were detached, seeded and transfected and treated for luciferase assay as described above.

Flachsbart F., Dose J., Gentschew L., Geismann C., Caliebe A., Knecht C., Nygaard M., Badarinarayan N., ElSharawy A., May S., Luzius A., Torres G.G., Jentzsch M., Forster M., Häsler R., Pallauf K., Lieb W., Derbois C., Galan P., Drichel D., Arlt A., Till A., Krause-Kyora B., Rimbach G., Blanché H., Deleuze J.F., Christiansen L., Christensen K., Nothnagel M., Rosenstiel P., Schreiber S., Franke A., Sebens S, & Nebel A. (2017). Identification and characterization of two functional variants in the human longevity gene FOXO3. Nature Communications, 8, 2063.

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