S1000 pcr thermal cycler

Manufactured by Bio-Rad

Sourced in United States

The S1000 PCR Thermal Cycler is a laboratory instrument designed for performing polymerase chain reaction (PCR) amplification of DNA samples. The device precisely controls the temperature and timing of the thermal cycling process essential for the PCR technique.

Automatically generated - may contain errors

Lab products found in correlation

Blood genomic dna kit, by Tiangen Biotech (1 mentions)

Spelling variants of this product

Thermal cycler (533 citations) S1000 Thermal Cycler (323 citations) S1000 (94 citations) PCR thermal cycler (33 citations) S1000 thermal cycler real-time PCR system (6 citations) Bio-Rad cyclers (3 citations) S1000 cycler (2 citations)

2 protocols using s1000 pcr thermal cycler

Degenerate Primers for Insect CYP6 Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four pairs of degenerate primers (dpE, dpF, dpG, and dpH) were designed according to the conserved amino acid sequences of insect CYP6 genes, and another pair of degenerate primers (dpA) reported by
Kasai et al. (1998) (link)
were taken for comparison (
Table 1). The amplification was performed with S1000 PCR Thermal Cycler (Bio-Rad,
www.bio-rad.com) using 2X ES Master mix Taq polymerase (CWBIO,
www.cwbiotech.com). The total reaction volume was 25 µL, including 1 µL cDNA, 1 uL 10 µM/L forward and reverse primer respectively, 12.5 µL 2X ES Master mix Taq polymerase, and 9.5 µL ddH2O. The PCR program was conducted under the following conditions: an initial denaturation at 94°C for 1 min followed by 30 cycles of 94°C for 30 sec, 45°C for 30 sec, and 72°C for 1 min, and a final extension at 72°C for 10 min. The PCR products were then separated by 1.5% agarose gel electrophoresis and stained with ethidium bromide (EB). The bands of the expected size (250 bp, 270 bp, and 420 bp) were excised and recovered using Universial DNA Purification Kit (Tiangen Biotech).

Wang H.D., Li F.F., He C., Cui J., Song W, & Li M.L. (2014). Molecular cloning and sequence analysis of novel cytochrome P450 cDNA fragments from Dastarcus helophoroides. Journal of Insect Science, 14, 28.

+ Open protocol

+ Expand

Genomic DNA Extraction and SNP Genotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was isolated from whole blood using the Blood Genomic DNA Kit (Tiangen Biotech, Beijing, China) according to the manufacturer protocols. Genotypes of SNP sites in the target genes were detected by direct sequencing of the polymerase chain reaction (PCR) product. The amplification reaction was performed in the S-1000 PCR Thermal Cycler (Bio-Rad, Hercules, CA, USA). The primer sequences used in this study are listed in Table 1. The PCR conditions were set as follows: an initial melting step at 94°C for 3 min, 35 cycles of denaturation at 94°C for 30 s, annealing at 58°C for 30 s, and extension at 72°C for 45 s, and a final elongation step at 72°C for 7 min. The PCR products were directly sequenced using an ABI 3500xL automated sequencer (Applied Biosystems, Foster City, CA, USA).

Li H.Y., Liu Y.C., Bai Y.H., Sun M., Wang L., Zhang X.B, & Cai B. (2016). SNP at miR-483-5p-binding site in the 3'-untranslated region of the BSG gene is associated with susceptibility to esophageal cancer in a Chinese population. Genetics and molecular research : GMR, 15(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!