Mouse anti gm130

Manufactured by Merck Group

Sourced in Canada

Mouse anti-GM130 is a monoclonal antibody that recognizes the GM130 protein, a membrane-bound protein found in the Golgi complex of mammalian cells. It is commonly used as a marker for the Golgi apparatus in immunofluorescence and other cell biology applications.

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Lab products found in correlation

Anti mouse cy3, by Merck Group (1 mentions) Anti rabbit cy3, by Merck Group (1 mentions) Mouse anti β actin, by GenScript (1 mentions) Rabbit anti lc3b, by Merck Group (1 mentions) Mouse anti eea1, by BD (1 mentions) Alexa fluor secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti zo 1, by Thermo Fisher Scientific (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rat anti e cadherin, by Merck Group (1 mentions) Rabbit and mouse anti claudin 2, by Thermo Fisher Scientific (1 mentions) Rabbit and mouse anti occludin, by Thermo Fisher Scientific (1 mentions) Mouse anti claudin 4, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

GM130 (6 citations) Anti‐GM130 (2 citations)

2 protocols using mouse anti gm130

Antibody Panel for WB and IF

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For Western blot (WB) and immnufluorescence, we employed: rabbit anti-LRP1β (Sigma–Aldrich Canada), rabbit anti-LC3B (Sigma–Aldrich), mouse anti-β-actin (GenScript), mouse anti-GM130 (Sigma), anti-rabbit HRP (Sigma), mouse anti-HRP (Sigma), Alexa Flour 488 anti-rabbit (Sigma), Cy3 anti-rabbit (Sigma), Cy3 anti-mouse, and Alexa Flour 594 anti-rabbit (Sigma).

Grosso R.A., Caldarone P.V., Sánchez M.C., Chiabrando G.A., Colombo M.I, & Fader C.M. (2019). Hemin induces autophagy in a leukemic erythroblast cell line through the LRP1 receptor. Bioscience Reports, 39(1), BSR20181156.

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Immunofluorescence Staining of Tight Junctions

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Cell culture reagents were obtained from Mediatech Inc, Manassas, VA. The following antibodies were used: rabbit anti-GOPC (Millipore, Billerica, MA), anti-E-Cadherin (Cell Signaling, Danvers, MA), mouse anti-EEA1 (BD Biosciences, San Jose, CA), mouse anti-TGN38 (ABR Affinity Bioreagents, Golden, CO). Rat anti-E-Cadherin, mouse anti-G58K, mouse anti-GM130 and mouse anti-β-actin were from Sigma (Saint Louis, MO). Rabbit and mouse anti-claudin-1, rabbit and mouse anti-claudin-2, rabbit and mouse anti-occludin, mouse anti-claudin-4, mouse anti-ZO-1 were from Invitrogen (Grand Island, NY). Mouse anti-JAM-A was a gift from Dr. Charles Parkos, Emory University. AlexaFluor secondary antibodies were also from Invitrogen. All other chemicals and reagents were from Sigma-Aldrich unless otherwise specified.

Lu R., Stewart L, & Wilson J.M. (2015). The scaffolding protein GOPC regulates tight junction structure. Cell and tissue research, 360(2), 321-332.

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