Cd138 clone b a38

Manufactured by Beckman Coulter

Sourced in United States, France

The CD138 (clone B-A38) is a monoclonal antibody that specifically recognizes the CD138 antigen, also known as syndecan-1. CD138 is a cell surface proteoglycan expressed on plasma cells and some other cell types. The CD138 (clone B-A38) antibody can be used for the identification and enumeration of plasma cells and other CD138-positive cells in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Ki67 clone b56, by BD (1 mentions) Pacific blue, by BD (1 mentions) Irf4 clone irf4.3e4, by BioLegend (1 mentions) Il 10, by Thermo Fisher Scientific (1 mentions) Il 15, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Cd45 clone hi30, by BD (1 mentions) Cyan adp, by Beckman Coulter (1 mentions) Igm clone g20 127, by BD (1 mentions)

3 protocols using cd138 clone b a38

Immunophenotyping of Hematological Cells

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Human recombinant interleukin (IL)-2 was purchased from R&D Systems (Minneapolis, MN, USA), interferon-alpha-2b (IFN- α, IntronA) from Merck Canada Inc. (Kirckland, Canada), IL-6, IL-10 and IL-15 from PeproTech (Rocky Hill, NJ, USA). Mouse monoclonal antibodies (mAbs) conjugated to allophycocyanin (APC), fluorescein isothiocyanate (FITC), peridinin chlorophyll protein-cyanin 5.5 (PerCP-Cy5.5), phycoerythrin (PE) or Pacific Blue^™ (PB), and specific for human CD19 (clone HIB19), CD24 (clone ML5), CD27 (clone M-T271), CD30 (clone BerH8), CD31 (clone WM59), CD38 (clone HIT2), CD56 (cloneB159), CD117 (clone 104D2), CD138 (clone RF8B2), IgG (clone G18-145), IgM (clone G20-127), lambda Ig light chain (clone JDC-12), kappa Ig light chain (clone TB28-2) and Ki67 (clone B56) were purchased from BD Biosciences (Le Pont De Claix, France); for CD200 (clone OX104) from eBiosciences (San Diego, CA, USA); for CD20 (clone B9E9), CD45 (clone J33), CD126 (clone M91) and CD138 (clone B-A38) from Beckman Coulter (Fullerton, CA, USA); for IRF4 (clone IRF4.3E4) and FCRL4 (clone 580810) from BioLegend (San Diego, CA, USA); for IgA, IgM, and IgG (polyclonal goat Abs) from Southern Biotech (Birmingham, AL, USA).

Jourdan M., Cren M., Schafer P., Robert N., Duperray C., Vincent L., Ceballos P., Cartron G., Rossi J.F., Moreaux J., Chopra R, & Klein B. (2016). Differential effects of lenalidomide during plasma cell differentiation. Oncotarget, 7(19), 28096-28111.

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Flow Cytometric Analysis of CSF and Blood Immune Cells

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We performed flow cytometric analysis of CSF and blood immune cells for 348 and 301 treatment-naive patients, respectively, as described previously.^{14 (link)} Flow cytometry data were available only for patients treated at the Klinikum rechts der Isar. We used the following antibodies for staining of B cells and plasmablasts: CD45 (clone HI30, BD Biosciences), CD19 (clone J3.119, Beckman Coulter), and CD138 (clone B-A38, Beckman Coulter) and analyzed the stained cells using a flow cytometer (CyAn ADP, Beckman Coulter). We then gated the cells on CD45 to select all leukocytes and subsequently on CD19 (CD45⁺ CD19⁺ B cells) and CD138 (CD45⁺ CD19⁺ CD138⁺ plasmablasts). We determined cell numbers using FlowJo v10 (FlowJo LLC) and calculated percentages of B cells and plasmablasts of all CD45⁺ cells.

Gasperi C., Andlauer T.F., Keating A., Knier B., Klein A., Pernpeintner V., Lichtner P., Gold R., Zipp F., Then Bergh F., Stangel M., Tumani H., Wildemann B., Wiendl H., Bayas A., Kümpfel T., Zettl U.K., Linker R.A., Ziemann U., Knop M., Warnke C., Friese M.A., Paul F., Tackenberg B., Berthele A, & Hemmer B. (2020). Genetic determinants of the humoral immune response in MS. Neurology® Neuroimmunology & Neuroinflammation, 7(5), e827.

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Multiparameter Flow Cytometry Phenotyping

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Human recombinant IL-2 was purchased from R&D Systems (Minneapolis, MN), IL-6 and IL-15 from AbCys SA (Paris, France), IL-10 from Peprotech (Rocky Hill, NJ), and IFN-α(Introna) from Merck Canada Inc. (Kirckland, Canada). Mouse mAbs conjugated to allophycocyanin (APC), fluorescein isothiocyanate (FITC), Pacific Blue (PB), peridinin chlorophyll protein-cyanin 5.5 (PerCP-Cy5.5), phycoerythrin (PE), BD Horizon Brilliant™ Blue 515 (BB515) against human CD1c (clone F10/21A3), CD19 (clone HIB19), CD22 (clone HIB22), CD24 (clone ML5), CD27 (clone M-T271), CD30 (clone BerH8), CD32 (clone FL18.26), CD38 (clone HIT2), CD40 (clone MAB89), CD72 (clone J4-117), CD196 (CCR6, clone 11A9), CD307c (FCRL3, clone H5) and immunoglobulin (Ig) -M (clone G20-127) were purchased from BD Biosciences (Le Pont De Claix, France); antibodies against CD11c (clone BU15), CD20 (clone B9E9), CD21 (clone BL13), CD126 (IL-6R, clone M91), and CD138 (clone B-A38) from Beckman Coulter (Fullerton, CA); antibodies against CD307d (FCRL4, clone H413D12) from Biolegend (San Diego, CA). Polyclonal goat Abs against human IgA and IgG were from Southern Biotech (Birmingham, AL).

Jourdan M., Robert N., Cren M., Thibaut C., Duperray C., Kassambara A., Cogné M., Tarte K., Klein B, & Moreaux J. (2017). Characterization of human FCRL4-positive B cells. PLoS ONE, 12(6), e0179793.

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