For all analytes, sample pretreatment was performed by a one-step liquid-liquid extraction procedure. A 100 µL aliquot of sample was transferred to a 10 mL glass centrifuge tube prior to adding 3 mL extraction solvent (ethyl acetate : isopropanol = 19 : 1, vol : vol). After 3 min of vortex-mixing, tubes were centrifuged at 1710 × g for 10 min at room temperature. Then, 2.7 mL of organic phase was drawn and transferred to 5 mL plastic centrifuge tubes and evaporated by a SCANVAC Freeze Dryer (Labogene, Shanghai, China) at 1000 × g, 73 bar, and room temperature for 30 min. The residual was reconstituted with 100 µL of 10% methanol aqueous solution, and 5 µL of the reconstituted solution was injected directly to the UHPLC-MS/MS system for analysis.
Scanvac freeze dryer
Manufactured by Labogene
Sourced in Denmark
The SCANVAC Freeze Dryer is a laboratory equipment designed for the lyophilization process. It removes water from a frozen sample through the process of sublimation, wherein the solid ice directly converts into water vapor without going through the liquid phase. The SCANVAC Freeze Dryer is capable of handling a range of sample volumes and can maintain precise temperature and pressure conditions required for the freeze-drying process.
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Lab products found in correlation
3 protocols using scanvac freeze dryer
1
Liquid-Liquid Extraction for UHPLC-MS/MS Analysis
2
Echis carinatus Venom Extraction Protocol
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Echis carinatus venom from Tamil Nadu was procured from Irula Cooperative Society, Tamil Nadu. Echis carinatus from Goa and Rajasthan were captured from their habitat (see Supplementary material S1, Table 1 ). Venom was milked by allowing snakes to bite the membrane fastened on the glass beaker (Mirtschin et al., 2006 (link)), and pooled for the region. It was transported in dry ice and lyophilized in ScanVac Freeze dryer (Labogene, Denmark).
3
Plasma Lipid Extraction and QC
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A 0.8-mL aliquot of mixed extractant composed of chloroform and methanol (1:3, V:V) was added to 0.2 mL of plasma sample, and the resultant sample was mixed vigorously and stored overnight at –20°C. The next day, each mixture was subjected to centrifugation at 13,000 × g at 4°C for 10 min, and 100 μL supernatant was transferred to a 1.5-mL tube and evaporated to dryness in a SCANVAC Freeze Dryer (Labogene, Shanghai, China) at 1000 × g, 73 bar, and room temperature for 1 h. The residue was reconstituted with 0.2 mL methanol and vigorous mixing for 1 min, and the supernatant was collected after centrifugation again. To prepare the quality-control (QC) sample, a 10-μL aliquot was collected from every processed sample and mixed. The QC sample was utilized to normalize deviations of measurement.
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