The primary human cell lines HUVEC, HUASMC and hPC-PL were purchased from PromoCell. EA.hy926, a cell line created by fusion of the A149 human lung carcinoma and the HUVEC cell line, and the human glioblastoma lines LN229 and A172, were purchased from American Type Culture Collection (ATCC). The human fibroblast cell line VH10T is a diploid telomerase-immortalized line, which was a kind gift from Prof. L. Mullenders, Leiden. The primary cell lines were cultured in endothelial cell growth medium 2, smooth muscle cell growth medium 2 and pericyte growth medium, purchased from PromoCell (Heidelberg, Germany), respectively. EA.hy926, LN229 and A172 were cultured in DMEM or DMEM GlutaMax (Gibco, Life Technologies Corporation, Paisley, UK) supplemented with 10% fetal calf serum (FCS; Gibco, Life Technologies Corporation, Paisley, UK), and for EA.hy926 with 1% HAT. All cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2. To ensure exponential growth during the whole experiment period, cells were seeded 48 h prior to treatment, and cell densities were chosen accordingly.
Ea hy926
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom
The EA.hy926 is a cell line derived from human umbilical vein endothelial cells. It is commonly used in in vitro research applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (9 mentions)
Ea hy926, by American Type Culture Collection (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Human umbilical vein endothelial cells (huvec), by PromoCell (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Mcf 7, by Thermo Fisher Scientific (2 mentions)
Endothelial cell growth medium, by Provitro (1 mentions)
Hdmec, by PromoCell (1 mentions)
Human umbilical vein endothelial cells (huvec), by Provitro (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Ea hy926 endothelial cells, by American Type Culture Collection (1 mentions)
Glutamine, by Sartorius (1 mentions)
Mkn45, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
15 protocols using ea hy926
1
Cell Culture Conditions for Diverse Cell Lines
2
Endothelial and Angiosarcoma Cell Lines
3
miRNA-93 Regulation of Endothelial Cell Hypoxia
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EA.hy926 endothelial cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc.), 10% fetal calf serum (Gibco; Thermo Fisher Scientific, Inc.), 1% glutamine, 1% nonessential amino acids, and penicillin (100 U/ml) at 37°C and 5% CO2 under humidified conditions. The cells were cultured in hypoxic conditions when the oxygen concentration of the incubator was adjusted to 1%. EA.hy926 cells (2×106/ml) cultured in the normal and hypoxic conditions were respectively transfected with miRNA-93 mimics using RNAiMAX Reagent (Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's instructions. Oligonucleotide transfection RNA oligos were chemically synthesized and purified by Shanghai Genepharma Co. Ltd. (Shanghai, China). The following rno-mir-93 agomir sequences were used: Sense, 5′-CAAAGUGCUGUUCGUGAGGUAG-3′ and antisense, 5′-ACCUGCACGAACAGCACUUUGUU-3′. The sequences of the rno-mir-93 agomir negative control used were as follows: Sense, 5′-UUCUCCGAACGUGUCACGUTT-3′ and antisense 5′-ACGUGACACGUUCGGAGAATT-3′. The final concentration of miRNA mimics used for transfection was 100 nM, and the cells were harvested at 12 h for subsequent experimentation.
4
Culture of EA.hy926 Endothelial Cells
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Human umbilical vein endothelial cell line, EA.hy926 was purchased from American Type C u l t u r e C o l l e c t i o n ( AT C C ) a n d c u l t u r e d a s previously reports (Li et al., 2015) . Briefly, EA.hy926 cells were cultured in Dulbecco's modified Eagle medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), penicillin (100 U/mL, Thermo Fisher Scientific), and streptomycin (100 U/mL, Thermo Fisher Scientific). The cultures were maintained at 37°C in a 95% humidified atmosphere with 5% CO 2 .
5
Culturing Gastric Cancer and Endothelial Cells
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The human gastric cancer-derived cell lines AGS (CRL-1739) and MKN-45 (JCRB0254) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and JCRB Cell Bank (Osaka, Japan), respectively. The human immortalized gastric cell line GES-1 was kindly gifted by Dr. Dawit Kidane (The University of Texas at Austin, USA). The immortalized human umbilical vein cell line EA.hy926 (CRL-2922) was obtained from ATCC. AGS and MKN-45 cells were cultured in RPMI 1640 medium, GES-1 cells were cultured in DMEM high glucose medium (ThermoFisher Scientific, Waltham MA, USA), and EA.hy926 cells were cultured in DMEM Ham F12 without red phenol (Thermo Fisher Scientific). All of them were supplemented with 2 mM glutamine, 10% fetal bovine serum (Biological Industries, Beit-Hamek, Israel), and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin) in a humidified atmosphere with 5% CO2 at 37 °C.
6
Cell Lines for In Vitro Experiments
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Human cell lines, HCT 116 (colorectal carcinoma), MCF-7 (hormone-sensitive breast cancer), and EA.hy926 (human endothelial cell), were obtained from ATCC, USA. Cells were cultured at a humidified atmosphere (37°C) with CO2 (5%) in a growth medium supplemented with 10% FBS and penicillin/streptomycin (1%). MCF-7 and EA.hy926 cells were cultured in DMEM (Gibco/Life technology, UK), while HCT-116 cells were cultured in RPMI-1640 (Sigma-Aldrich, USA).
7
Culturing EAhy926 and NIH 3T3 Cells
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EAhy926 and murine NIH 3T3 cells were purchased from ATCC (LGC Standards GmbH, Wesel, Germany), and ATCC (Manassas, VA, USA), respectively. Passages P3-14 were used for all experiments. EAhy926 cells were grown in DMEM (Gibco, Thermo Fisher Scientific, Reinach, Switzerland) containing 10% fetal bovine serum at 37 °C, 5% CO2, 95% humidity. NIH 3T3 mouse fibroblasts were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 10% bovine calf serum and 1% penicillin-streptomycin, at 37 °C under an atmosphere of humidified air containing 5% CO2.
8
Cell Culture Protocols for Various Cell Lines
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NCL-H292 [H292] (#SCSP-582), HEK293T (#SCSP-502), EA.hy926 (#GNHu39) and Lewis lung cancer (LLC, #TCM 7) cell lines were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). All the cells were cultured in a humidified atmosphere of 5% CO2 at 37 °C. H292 cells were grown in RPMI-1640 (Gibico, New York, USA) medium supplemented with 10% fetal bovine serum (FBS) (HyClone, Utah, USA) and 100 U/ml Penicillin-Streptomycin (Gibico, USA). HEK293T, EA.hy926 and LLC cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, USA) supplemented with 10% FBS and 100 U/ml Penicillin-Streptomycin.
9
Culturing EA.hy926 Human Vein Cells
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The EA.hy926 cells, primary human umbilical vein cell line, were derived from the American Type Culture Collection (ATCC). The accession number of EA.hy926 from ATCC is CVCL_3901 (Homo sapiens (Human)). The EA.hy926 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose medium, cat no: 12800-058) supplement with 10% foetal bovine serum (FBS), 1% of 100 units/mL penicillin/streptomycin and 1% of 2 mmol/L l -glutamine (Gibco, Gaithersburg, MA) at 37 °C in incubate with 5% CO2 until 70–80% confluence for further experiments.
10
Umbilical Vein Cell Inflammatory Response
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EA.hy926 (human umbilical vein cell fusion cell) cells were purchased from the American Type Culture Collection (ATCC). EA.hy926 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) containing 10% FBS and 1%antibiotics (penicillin–streptomycin) in a CO2 incubator (5% CO2) at 37 °C. The cells were subsequently starved for 12 h in serum-free medium before the experiment. Cells were divided into the control, LPS (LPS 1000 μg/mL), FL (LPS 1000 μg/mL + FGF-21 0.1 M), and FH (LPS 1000 μg/mL + FGF-21 1.0 M) groups. After treatment, cells in each group were cultured for an additional 12 h.
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