Sc 101199

Manufactured by Proteintech

SC-101199 is a laboratory equipment product. It serves a core function related to laboratory applications, however, a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Alexa flour 647, by Thermo Fisher Scientific (1 mentions) Ab125189, by Abcam (1 mentions) Lsm 710, by Zeiss (1 mentions) Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 647, by Thermo Fisher Scientific (1 mentions)

2 protocols using sc 101199

Immunofluorescence Staining of MDAMB231 Cells

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4% paraformaldehyde was used to fix MDAMB231 cells for 15 min, 0.25% Triton X-100 to permeabilize them for 15 min, and 3% BSA to block them for 1 h at room temperature. The rabbit anti-RNF31 antibody (ab125189, Abcam, 1:400), mouse anti-YAP antibody (SC-101199, 1:200) and mouse anti-PD-L1 antibody (1:400, 66,248–1-Ig, Proteintech) were used. The Alexa Flour 647 (Invitrogen) anti-mouse or Alexa Flour 488 anti-rabbit secondary antibodies (Invitrogen). The nuclei were stained with DAPI (Beyotime). Samples with only secondary antibodies and no primary antibodies were used as negative controls. Images were captured with a Nikon A+ laser scanning confocal system.

Yang H., Xue M., Su P., Zhou Y., Li X., Li Z., Xia Y., Zhang C., Fu M., Zheng X., Luo G., Wei T., Wang X., Ding Y., Zhu J, & Zhuang T. (2022). RNF31 represses cell progression and immune evasion via YAP/PD-L1 suppression in triple negative breast Cancer. Journal of Experimental & Clinical Cancer Research : CR, 41, 364.

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Immunostaining for Cytoskeleton and Mechanosensitive Proteins

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Samples were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature and permeabilized with 0.05% Triton X-100 in PBS for 15 min. They were then blocked with 1% BSA in PBST for 1 h, before incubated with primary antibodies against Yes Associated Protein (YAP) (1:200, mouse, Santa Cruz sc-101199) and Piezo1 (1:200, Rabbit, Proteintech 15939-1-AP), or α-SMA (1:200, rabbit, Invitrogen) and Vimentin (1:200, mouse, Sigma) overnight at 4 °C. After rinse, the samples were incubated with secondary antibodies (1:200; AlexaFluor-555/647; Invitrogen) at room temperature for 1 h. The cytoskeleton was stained by AlexaFluor 488 conjugated phalloidin (Invitrogen), and cell nuclei were labeled by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Confocal images were taken by Zeiss LSM 710.

Wang M., Sun S., Dong G., Long F, & Butcher J.T. (2023). Soft, strong, tough, and durable protein-based fiber hydrogels. Proceedings of the National Academy of Sciences of the United States of America, 120(8), e2213030120.

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