Analysis of TH- and DAT-positive fibers in the striatum was performed under blinded conditions. The density of TH- and DAT-positive fibers in the dorsal striatum was measured bilaterally from 3 coronal sections of each rat at the approximate rostro-caudal levels of AP=+1.2; +0.5 and -0.3 mm, relative to the bregma, according to the rat brain atlas [30 ]. Digital images of the immunolabelled sections were acquired with an automated bright field microscopy slide scanner (3DHistech Ltd., Hungary). Obtained images were converted to 8-bit grey scale, after which the colours were inverted and the integrated optical densities of the dorsal striata divided by the measured area were analyzed with Fiji ImageJ software (Media Cybernetics Inc, USA). Nonspecific background staining was measured from corpus callosum and subtracted from the striatal optical densities. The data are presented as percentage of the lesioned striatum as compared to the intact striatum.
Fiji imagej software
Manufactured by Media Cybernetics
Sourced in United States
Fiji ImageJ software is an open-source image processing program developed for scientific image analysis. It provides a platform for working with a variety of image formats and includes a comprehensive set of tools for image processing, analysis, and visualization.
Automatically generated - may contain errors
3 protocols using fiji imagej software
1
Striatal Fiber Density Analysis
2
Quantifying Striatal Dopaminergic Denervation
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Optical density of the TH-ir and DAT-ir fibers in the dorsal striatum was measured bilaterally from 3 different rostro-caudal levels through the striatum (approximately A/P + 1.2, + 0.48, and –0.26, mm relative to the bregma [55 ]). The total magnitude of striatal denervation was analyzed as an average reduction in the optical density at the three levels measured. Digital images of the TH or DAT-immunostained sections were acquired with Pannoramic P250 Flash II whole slide scanner (3DHistech). The images were converted to 8-bit gray scale, and colors were inverted. The dorsal part of caudate putamen limiting to the ventral edge of external capsule of corpus callosum was outlined. From this outlined area, integrated optical density divided by the size of the area was measured with Fiji ImageJ software (Media Cybernetics, USA) by a blinded observer. Optical density of nonspecific background staining was measured from corpus callosum and subtracted from the striatal optical densities. The data are presented as percentage of the lesioned striatum as compared to the intact striatum.
3
Striatal Fiber Density Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analysis of TH- and DAT-positive fibers in the striatum was performed under blinded conditions. The density of TH- and DAT-positive fibers in the dorsal striatum was measured bilaterally from 3 coronal sections of each rat at the approximate rostro-caudal levels of AP=+1.2; +0.5 and -0.3 mm, relative to the bregma, according to the rat brain atlas [30 ]. Digital images of the immunolabelled sections were acquired with an automated bright field microscopy slide scanner (3DHistech Ltd., Hungary). Obtained images were converted to 8-bit grey scale, after which the colours were inverted and the integrated optical densities of the dorsal striata divided by the measured area were analyzed with Fiji ImageJ software (Media Cybernetics Inc, USA). Nonspecific background staining was measured from corpus callosum and subtracted from the striatal optical densities. The data are presented as percentage of the lesioned striatum as compared to the intact striatum.
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