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L tryptophan

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L-Tryptophan is a naturally occurring amino acid used in various lab applications. It serves as a precursor for the synthesis of serotonin and other important biomolecules. L-Tryptophan is available in high purity for use in research and development settings.

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Lab products found in correlation

Fumaric acid, by Cambridge Isotopes (2 mentions) Nicotinic acid, by Cambridge Isotopes (2 mentions) Deuterated picolinic acid, by Thermo Fisher Scientific (2 mentions) Dl glutamic acid, by Cambridge Isotopes (2 mentions) Acetonitrile acn, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by G Biosciences (1 mentions) L lysine, by Cambridge Isotopes (1 mentions) Protein assay dye regent, by Bio-Rad (1 mentions) L glutamine, by Cambridge Isotopes (1 mentions) L methionine, by Cambridge Isotopes (1 mentions) L glutamic acid, by Cambridge Isotopes (1 mentions) Aav8 tbg cre, by Addgene (1 mentions) 0.45 μm nitrocellulose membrane, by Bio-Rad (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) N methyl n trimethylsilyl trifluoroacetamide, by Merck Group (1 mentions) L valine, by Cambridge Isotopes (1 mentions)

3 protocols using l tryptophan

1

Quantitative Metabolite Analysis Protocol

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Formic acid (FA), methanol (MeOH), dimethylsulfoxid (DMSO) and acetonitrile (ACN) of analytical grade were from Fisher Scientific (Pittsburgh, PA, USA). Water was of ultrapure grade (EMD Millipore Co., Billerica, MA, USA). Deuterated standards {Fumaric acid (2,3-D2, 98% purity), DL-Glutamic acid (2,4,4-D3, 98% purity), L-Tryptophan (indole-D5, 98% purity) and Nicotinic acid (D4, 98% purity)} were from Cambridge Isotope Laboratories (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA). Deuterated Anthranilic acid (3,4,5,6-D4, 98% purity), Kynurenic acid (3,4,6,7,8-D5, 98% purity), Kynurenine (D4, 98% purity), Quinolinic acid (4,5,6-D3, 99% purity), 3-Hydroxy-DL-kynurenine (5,8,8-D3, 98% purity), 3-Hydroxyanthranilic acid (4,6-D2, 98% purity) were from Buchem (Buchem BV, Apeldoorn, The Nederlands). Deuterated Picolinic acid (99% purity) was from Acros Organics (Thermo Fisher Sci, NJ, USA). Commercial calibration solutions for the MS device were from Thermo Scientific (Thermo Fisher Sci., San José, CA, USA).
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
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2

Metabolomic Analysis of Transgenic Mice

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C57bl6/j mice were obtained from Jackson Laboratories (Stock #000664). Labeled internal standards citric acid (2,2,4,4-D4, 98%; #DLM-3487), succinic acid (2,2,3,3-D4, 98%; #DLM-584), l-valine (2,3,4,4,4,5,5,5-D8; 98%; #DLM-311), l-glutamic acid (13C5, 99%; #CLM-1800), l-glutamine (13C5, 99%, #CLM-1822), l-lysine (13C6, 99%; #CLM-2247), l-methionine (13C5, 99%; #CLM-893), and l-tryptophan (13C11, 99%; #CLM-4290) and tracer glucose (13C6, 99%, #CLM-1396) were obtained from Cambridge Isotope Laboratories, Inc. AAV8-TBG-NULL (#105536-AAV8) and AAV8-TBG-Cre (#107787-AAV8) were purchased from Addgene. Pre-filled bead mill tubes containing 1.4 mm ceramic beads (#15-340-153) were purchased from FisherScientific. Methoxaymine hydrochloride (#226904, MOX) and N-methyl-N-(trimethylsilyl) trifluoroacetamide (#694709, MSTFA) were purchased from Sigma Aldrich. Protease inhibitor cocktail (#786-437) was purchased from G Biosciences. Bio-Rad protein assay dye regent (#5000006) and 0.45 μm nitrocellulose membrane (#1620115) were purchased from Bio-Rad.
Rauckhorst A.J., Borcherding N., Pape D.J., Kraus A.S., Scerbo D.A, & Taylor E.B. (2022). Mouse tissue harvest-induced hypoxia rapidly alters the in vivo metabolome, between-genotype metabolite level differences, and 13C-tracing enrichments. Molecular Metabolism, 66, 101596.
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3

Quantitative Metabolite Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formic acid (FA), methanol (MeOH), dimethylsulfoxid (DMSO) and acetonitrile (ACN) of analytical grade were from Fisher Scientific (Pittsburgh, PA, USA). Water was of ultrapure grade (EMD Millipore Co., Billerica, MA, USA). Deuterated standards {Fumaric acid (2,3-D2, 98% purity), DL-Glutamic acid (2,4,4-D3, 98% purity), L-Tryptophan (indole-D5, 98% purity) and Nicotinic acid (D4, 98% purity)} were from Cambridge Isotope Laboratories (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA). Deuterated Anthranilic acid (3,4,5,6-D4, 98% purity), Kynurenic acid (3,4,6,7,8-D5, 98% purity), Kynurenine (D4, 98% purity), Quinolinic acid (4,5,6-D3, 99% purity), 3-Hydroxy-DL-kynurenine (5,8,8-D3, 98% purity), 3-Hydroxyanthranilic acid (4,6-D2, 98% purity) were from Buchem (Buchem BV, Apeldoorn, The Nederlands). Deuterated Picolinic acid (99% purity) was from Acros Organics (Thermo Fisher Sci, NJ, USA). Commercial calibration solutions for the MS device were from Thermo Scientific (Thermo Fisher Sci., San José, CA, USA).
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
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