Primary antibodies used were polyclonal rabbit anti-NE81 [12 (link)], monoclonal rat YL1/2 directed against α-tubulin [35 (link)], monoclonal mouse 9E10 directed against myc-tag (EQKLISEEDL) [36 (link)], and monoclonal mouse Act-1 against Dictyostelium actin [37 (link)]. Secondary antibodies involved Alexa Fluor conjugates purchased from Life Technologies (Darmstadt, Germany), Atto conjugates from Atto-Tec GmbH (Siegen, Germany), and enzyme conjugates for Western blotting from Sigma (Deisenhofen, Germany).
Enzyme conjugates for western blotting
Manufactured by Merck Group
Sourced in Germany
Enzyme conjugates for Western blotting are laboratory reagents used to detect and quantify specific proteins in a sample. They consist of an enzyme, such as horseradish peroxidase or alkaline phosphatase, covalently linked to an antibody that binds to the target protein. These conjugates are used as part of the detection system in Western blotting techniques, allowing for the visualization and analysis of proteins separated by gel electrophoresis.
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Lab products found in correlation
2 protocols using enzyme conjugates for western blotting
1
Antibody Characterization Protocol
2
Comprehensive Immunostaining Protocol
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Primary antibodies used in this study: anti-Cep192 [17 (link)], anti-CP39 [13 (link)], anti-CP55 [14 (link)], anti-CP91 [15 (link)], anti-CP148 [16 (link)], anti-CDK5RAP2 [17 (link)], anti-CP224 [32 (link)], anti-NE81 [33 (link)], anti-α-tubulin [34 (link)]. Secondary antibodies and fluorescent Streptavidin were purchased from Thermo Fisher Scientific (Darmstadt, Germany), the Spot-Label nanobody recognizing the SpotH6-tag was obtained from Chromotek (Planegg-Wiedmannsried, Germany), and enzyme conjugates for Western blotting were from Sigma (Deisenhofen, Germany).
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