Log In Sign up
The largest database of trusted experimental protocols

Hifair 2 1st strand cdna synthesis supermix kit

Manufactured by Yeasen
Visit Supplier
Sourced in China

The Hifair II 1st Strand cDNA Synthesis SuperMix Kit is a laboratory product designed for the reverse transcription of RNA into complementary DNA (cDNA). The kit contains a proprietary enzyme blend and optimized buffer system to facilitate the efficient conversion of RNA into single-stranded cDNA, which can then be used for various downstream applications, such as PCR amplification.

Automatically generated - may contain errors

Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (3 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Cfx96, by Bio-Rad (2 mentions) Sybr green, by Yeasen (2 mentions) Rnaiso plus, by Takara Bio (2 mentions) Hieff qpcr sybr green master mix kit, by Yeasen (2 mentions) Hieff unicon universal blue qpcr sybr green master mix, by Yeasen (2 mentions) Chamq sybr qpcr master mix, by Vazyme (1 mentions) Hieff qpcr sybr green master mix, by Yeasen (1 mentions) Quantstudio 5 system, by Thermo Fisher Scientific (1 mentions) Rneasy kit, by Beyotime (1 mentions)

Spelling variants of this product

Hifair® II 1st Strand cDNA Synthesis Kit (114 citations) Hifair® II 1st Strand cDNA Synthesis SuperMix (65 citations) CDNA Synthesis SuperMix (22 citations) Hifair® 1st Strand cDNA Synthesis SuperMix (15 citations) 1st Strand cDNA Synthesis SuperMix (14 citations) Hifair 1st Strand cDNA Synthesis Kit (8 citations) CDNA Synthesis SuperMix Kit (5 citations) Hifair™ cDNA Synthesis Kit (4 citations) Hifair 1st Strand cDNA Synthesis SuperMix Kit (2 citations) Hifair cDNA Synthesis SuperMix kit (2 citations)

9 protocols using hifair 2 1st strand cdna synthesis supermix kit

1

Quantitative Assessment of TLR4 Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from collected lung tumor tissues using Trizol (Invitrogen) and reverse transcribed into cDNA by using HifairTM II 1st Strand cDNA Synthesis SuperMix Kit (11123ES60, YEASEN, China) according to the manufacturer’s protocol. Quantitative PCR reactions were performed by a BIO-RAD CFX96™ (Bio-rad, USA) with the supplement of SYBR Green (11201ES08*, YEASEN, Shanghai, China) and the amplification of the desired products was observed and recorded using CFX96TM Real-Time PCR Detection System. Reactions were performed in triplicate. The fold difference in transcripts was calculated using the ΔΔCt method with GAPDH as a control [31 (link)]. All the above primers were synthesized by Biomed Company (Beijing, China). The gene sequences used were as follows:

TLR4-F: 5’-ATGGCATGGCTTACACCACC-3’;

TLR4-R: 5’-GAGGCCAATTTTGTCTCCACA-3’;

GAPDH-F: 5’-AGGTCGGTGTGAACGGATTTG-3’;

GAPDH-R: 5’-TGTAGACCATGTAGTTGAGGTCA-3’.

Cui Y., Li Y., Long S., Xu Y., Liu X., Sun Z., Sun Y., Hu J, & Li X. (2023). Comprehensive analysis of the immunogenic cell death-related signature for predicting prognosis and immunotherapy efficiency in patients with lung adenocarcinoma. BMC Medical Genomics, 16, 184.
+ Open protocol
+ Expand
2

RNA Extraction and Real-Time qPCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted with TRIZOL reagent (Thermo Fisher, USA). The cDNA was prepared using HifairTM II 1st Strand cDNA Synthesis SuperMix Kit (YEASEN, Shanghai, China) according to the manufacturer’s protocol. Real time q-PCR was performed using ChamQ SYBR qPCR Master Mix (Vazyme, Nanjing, China) following instructions. 18S(Human) was used as an internal reference to normalize the expression of other mRNAs. Sequences used in this study were listed as follows:
Liu Q., Lian Q., Song Y., Yang S., Jia C, & Fang J. (2023). Identification of LSM family members as potential chemoresistance predictive and therapeutic biomarkers for gastric cancer. Frontiers in Oncology, 13, 1119945.
+ Open protocol
+ Expand
3

cDNA Synthesis and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cDNA was prepared using HifairTM II 1st Strand cDNA Synthesis SuperMix Kit (11123ES60, YEASEN, Shanghai, China) according to the manufacturer's protocol. PCR was performed by a BIO-RAD CFX96™ (Bio-rad, San Diego, USA) in the presence of SYBR Green (11201ES08*, YEASEN, Shanghai, China). A melting-curve analysis was performed after the fluorescence values were collected. The sequences of primers for the qRT-PCR analysis are listed in Table S1. All the above primers were synthesized by Sangon Biotech (Shanghai, China).
Ouyang S., Li H., Lou L., Huang Q., Zhang Z., Mo J., Li M., Lu J., Zhu K., Chu Y., Ding W., Zhu J., Lin Z., Zhong L., Wang J., Yue P., Turkson J., Liu P., Wang Y, & Zhang X. (2022). Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer. Redox Biology, 52, 102317.
+ Open protocol
+ Expand
4

Ovarian Cancer Transcriptome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from tissues of OV patients was extracted using RNAiso Plus (9108, TaKaRa, Japan), according to the manufacturer’s protocol. Following that, the Hifair II 1st Strand cDNA Synthesis SuperMix Kit (11123ES10,Yeasen, Shanghai, China) was used for reverse transcription of cDNA. qRT-PCR was conducted using Hieff qPCR SYBR Green Master Mix (11201ES03, Yeasen). GAPDH was used as an endogenous control for mRNA expression. Expression level analysis was performed using the 2−ΔΔCt method. All primer sequences used in this study are listed in Table S2.
Zhou M., Li B., Liu J, & Hong L. (2021). Genomic, Immunological, and Clinical Characterization of Pyroptosis in Ovarian Cancer. Journal of Inflammation Research, 14, 7341-7358.
+ Open protocol
+ Expand
5

Quantitative Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from CC and matched normal tissues, cells, and treated Siha and Hela cells using RNAiso Plus (Cat# 9108; TAKARA BIO INC) according to the manufacturer's protocol. A Hifair II 1st Strand cDNA Synthesis SuperMix kit (Yeasen) was used to synthesize cDNA from RNA according to the manufacturer's protocol. qRT‐PCR was performed using a Hieff qPCR SYBR Green Master Mix kit (Yeasen) in an ABI QuantStudio 5 according to the manufacturer's protocol. The mRNA expression levels of genes were measured using the 2−△Ct and 2−△△Ct method. GAPDH was used as the endogenous control to normalize RNA expression. The primer sequence for qRT‐PCR is shown in the Supplementary Material (primer sequence).
Zhu Y., Ren C., Jiang D., Yang L., Chen Y., Li F., Wang B, & Zhang Y. (2021). RPL34‐AS1–induced RPL34 inhibits cervical cancer cell tumorigenesis via the MDM2‐P53 pathway. Cancer Science, 112(5), 1811-1821.
+ Open protocol
+ Expand
6

Quantitative Gene Expression Analysis in Huh7 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from Huh7 cells using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. A total of 1 µg RNA was reverse transcribed into cDNA using the Hifair® II 1st Strand cDNA Synthesis SuperMix kit (Yeasen Biotech Co.), under the following conditions: 25°C for 5 min, 55°C for 15 min and 85°C for 5 min. qPCR was subsequently performed using the Hieff® qPCR SYBR Green Master Mix kit (Yeasen Biotech Co.) in QuantStudio™ 5 System (Thermo Fisher Scientific, Inc.). The following thermocycling conditions were used for qPCR: 95°C for 5 min, followed by 40 cycles at 95°C for 10 sec, 60°C for 30 sec and elongation at 72°C for 2 min. The following primer sequences were used for qPCR: SOCS2 forward, 5′-GAGCCGGAGAGTCTGGTTTC-3′ and reverse, 5′-ATCCTGGAGGACGGATGACA-3′; and GAPDH forward, 5′-GGTCTCCTCTGACTTCAACA-3′ and reverse, 5′-GTGAGGGTCTCTCTCTTCCT-3′. Relative mRNA levels were calculated using the 2−ΔΔCq method (25 (link)) and normalized to the internal reference gene GAPDH.
Liu J., Liu Z., Li W, & Zhang S. (2021). SOCS2 is a potential prognostic marker that suppresses the viability of hepatocellular carcinoma cells. Oncology Letters, 21(5), 399.
+ Open protocol
+ Expand
7

Quantitative RNA Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted from cells and tissues using an RNeasy Kit (R0027, Beyotime) according to the manufacturer’s instructions. Reverse transcription was completed using a Hifair II 1st Strand cDNA Synthesis SuperMix Kit (11123ES60, Yeasen). qRT‒PCR was performed on a real-time PCR system using SYBR dye (Yeasen). mRNA expression was normalized to that of GAPDH, which was used as the housekeeping gene. The relative expression levels of all the qRT‒PCR products were calculated using the ΔΔCt method27 (link). The list of primers used in this study is shown in Supplementary Table 2.
Yang S., Xie J., Pan Z., Guan H., Tu Y., Ye Y., Huang S., Fu S., Li K., Huang Z., Li X., Shi Z., Li L, & Zhang Y. (2024). Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop. Experimental & Molecular Medicine, 56(3), 630-645.
+ Open protocol
+ Expand
8

RNA Extraction and RT-qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) was used to extract total RNA from all Sham and SCI group. A Hifair™ II 1st-Strand cDNA Synthesis SuperMix Kit (Yeasen Biotechnology, Wuhan, China) was used to synthesize the cDNA. A Hieff UNICON® Universal Blue qPCR SYBR Green Master Mix (Yeasen Biotechnology) was used for real-time quantitative polymerase chain reaction (RT-qPCR), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was the endogenous control. The primer sequences used in this study are shown in Table S1.
Liu X., Li Z., Tong J., Wu F., Jin H, & Liu K. (2024). Characterization of the Expressions and m6A Methylation Modification Patterns of mRNAs and lncRNAs in a Spinal Cord Injury Rat Model. Molecular neurobiology.
+ Open protocol
+ Expand
9

Quantitative Real-Time PCR for Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted using TRIzol™ (Invitrogen, 15,596,018). Hifair™ II 1st Strand cDNA Synthesis SuperMix Kit (Yeasen, 11123ES60) was used for cDNA synthesis. Quantitative real-time PCR was performed with Hieff UNICON™ Universal Blue qPCR SYBR Green Master Mix (Yeasen, 11184ES08) according to the manufacturer’s protocol. Gene expression levels were normalized to GAPDH. Primers for real-time qPCR are listed in Additional file1: Table S1.
Liang L., Tian Y., Feng L., Wang C., Feng G., Stacey G.N., Shyh-Chang N., Wu J., Hu B., Li W., Hao J., Wang L, & Wang Y. (2022). Single-cell transcriptomics reveals the cell fate transitions of human dopaminergic progenitors derived from hESCs. Stem Cell Research & Therapy, 13, 412.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!