PEG hydrogels were formed using free radical-based photo-polymerization, which is the most common method used to prepare biomimetic hydrogels [81 (link)]. To obtain 50 μl of 5wt%. PEG-Ac, 5 μl of 500mg/ml 4 arm-Ac-PEG (Mw = 10kDa, Laysan Bio, Inc., USA) were mixed with 2.5 μl of 200 mg/ml protease degradable peptide cross-linker GCRDVPMSMRGGDRCG (Mw = 2kDa, Genscript, USA), 1 μl of 5mg/ml photoinitiator (Irgacure 2959, Sigma Aldrich, USA) and 7.5 μl PBS. The thiolated crosslinker was added at a 2:1 molar ratio of acrylate:thiol. The PEG-Ac solution was cast into a PDMS mold of 50 μl hydrogel using UV irradiation at an intensity of 5 mW/cm2 (OmniCureR Series 1500, Excelitas Technologies Ltd, USA) for 5 min.
Gcrdvpmsmrggdrcg
Manufactured by GenScript
Sourced in United States
The GCRDVPMSMRGGDRCG is a laboratory equipment product designed to facilitate specific research and analysis tasks. Its core function is to provide a controlled environment and tools for researchers to conduct their experiments. Further details about its intended use or specific applications are not available.
Automatically generated - may contain errors
Lab products found in correlation
Irgacure 2959, by Merck Group (3 mentions)
4 arm ac peg, by Laysan Bio (3 mentions)
Matrigel matrix, by Corning (1 mentions)
Human recombinant fgf2, by Thermo Fisher Scientific (1 mentions)
Grgdspc, by GenScript (1 mentions)
Hexa ethylene glycol dithiol, by Merck Group (1 mentions)
Grdgspc, by GenScript (1 mentions)
Grdgspc, by AAPPTec (1 mentions)
Gcrdipeslragdrcg, by GenScript (1 mentions)
7 protocols using gcrdvpmsmrggdrcg
1
Photopolymerized Biomimetic PEG Hydrogels
2
Biomimetic Brain Tissue Hydrogel
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brain tissue-mimicking hydrogel was prepared by interpenetrating growth-factor-reduced Matrigel matrix (Corning) and matrix metalloproteinase (MMP)-sensitive hyaluronic acid (HA) hydrogels with a volume ratio of 1:1. MMP-sensitive HA hydrogel was synthesized as described previously (Wang et al., 2019 (link)). Briefly, HA-ADH was firstly obtained using hyaluronic acid (100 mg, 0.0015 mmole, 50 kDa), ADH (2.6 g, 8.4 mmole) and 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) (0.3 g, 0.92 mmole) at pH 4.75, followed by dialysis (MWCO 6–8 kDa) in deionized water for 2–3 days and lyophilizing. Acrylated hyaluronic acid (HA-AC) was prepared by reacting the synthesized HA-ADH (100 mg, 0.0014 mmole) with N-Acryloxysuccinimide (NHS-AC) (108 mg, 0.47 mmole) in HEPES buffer overnight and dialysis in DI water for 2–3 days before lyophilizing. HA-AC was further conjugated with RGD peptides (Ac-GRGDSPCG-NH2, Genscript) overnight, dialysis in DI water for 2 days and lyophilizing. Finally, MMP-sensitive HA hydrogel (10 mg/mL) was obtained by crosslinking with MMP-degradable crosslinker (GCRDVPMSMRGGDRCG, Genscript).
3
Enhanced Muscle Stem Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four-arm PEG-4MAL macromer (molecular weight, 22,000 or 10,000; Laysan Bio) was dissolved in 1× phosphate-buffered saline (PBS) containing 10 mM Hepes (pH 7.4). Cell adhesive peptides (GRGDSPC, GRDGSPC, CGGEGYGEGYIGSR, and CGGKAFDITYVRLKF; >95% purity; GenScript) were dissolved in 1× PBS containing 10 mM Hepes and added to PEG-4MAL solution to produce functionalized PEG-4MAL precursors. Freshly isolated MuSCs were then added to the solution containing functionalized PEG-4MAL precursors. To synthesize cell-encapsulated hydrogels, the solution containing functionalized PEG-4MAL precursors and cells was mixed with protease-degradable cross-linking peptide (GCRDVPMSMRGGDRCG; GenScript) or nondegradable hexa(ethylene glycol) dithiol (Sigma-Aldrich) dissolved in 1× PBS containing 10 mM Hepes and subsequently polymerized at 37°C/5% CO2 for 5 min before adding MuSC growth media (F10 containing 1% penicillin/streptomycin, 1% GlutaMAX, and 20% horse serum). Recombinant human FGF-2 (25 ng ml−1; PeproTech) was supplemented daily. To prime the MuSCs to differentiate, the growth medium was replaced with differentiation media (DMEM containing 1% penicillin/streptomycin, 1% GlutaMAX, and 2% horse serum) on days 5 and 6 of culture. Cells were cultured in the differentiation media for an additional 4 days.
4
PEG-lysostaphin Hydrogel for Antimicrobial Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twenty-kilodalton PEG-4MAL macromer (Lysan Bio) was mixed with recombinant lysostaphin protein (AMBI Products LLC) in 100 mM MES buffer, pH 5.5–6.0. Hydrogels were then cross-linked in a one-step reaction by combining PEG-lysostaphin with either the GFOGER peptide, GGYGGP(GPP)5GFOGER(GPP)5GPC (New England Peptide), or the RGD peptide, GRDGSPC (AAPPTEC), VPM cross-linker, GCRDVPMSMRGGDRCG (Genscript), and the bacterial suspension. Bacterial suspensions were prepared by picking individual colonies of bacteria grown on a TSA plate overnight and suspending them in Dulbecco’s PBS supplemented with calcium and magnesium (PBS) to an optical density of 0.20 at 600 nm (MicroScan Turbidity Meter; Seimens) and then diluting this suspension 100-fold. The viable count for all bacterial inocula was determined by plate count on TSA medium. Unless otherwise noted the hydrogels were 4.0% wt/vol 20-kDa PEG-4MAL, 1 mM GFOGER, and 424 U/mL lysostaphin. The amount of VPM cross-linker added was determined stoichiometrically by matching the remaining maleimide groups after accounting for GFOGER or RGD incorporation. After mixing, the hydrogels were allowed to gel for 15 min in a humidified incubator at 37 °C and 5.0% CO2 for in vitro studies or polymerized over the fracture for in vivo studies.
5
3D PEG-Maleimide Hydrogel Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
3D PEG-maleimide (PEG-Mal) hydrogels were prepared at 20 wt. % with a 20 K 4-arm PEG-Mal (Jenkem Technology, Plano, TX) and crosslinked in 2 mM triethanolamine (pH ∼ 7.4) at a 1:1 molar ratio with 50% 1.5 K linear PEG-dithiol (Jenkem), ∼17% GCRDVPMSMRGGDRCG, ∼17% GCRDSGESPAYYTADRCG, and ∼17% GCRDIPESLRAGDRCG (GenScript, Piscataway, NJ). Cells were pelleted and resuspended in PEG-Mal solution before mixing with PEG-dithiol. The cell adhesion peptides (GenScript, Piscataway, NJ) CGP(GPP)5GFOGER(GPP)5 (1 mM), CGPHSRN(G)6RGDS (0.8 mM), CGGSVVYGLR (0.1 mM), and CGGAEIDGIEL (0.1 mM) were reacted with PEG-Mal 10 min before gelation. Gels were polymerized in 10 μl volumes for 5 min before swelling in cell culture medium.
6
PEG Hydrogel Formation via UV Photo-polymerization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PEG hydrogels using UV photo-polymerization PEG hydrogels were formed using free radical-based photo-polymerization, which is the most common method used to prepare biomimetic hydrogels [81] (link). To obtain 50µl of 5wt%. PEG-Ac, 5µl of 500mg/ml 4 arm-Ac-PEG (Mw = 10kDa, Laysan Bio, Inc., USA) were mixed with 2.5µl of 200 mg/ml protease degradable peptide crosslinker GCRDVPMSMRGGDRCG (Mw = 2kDa, Genscript, USA), 1µl of 5mg/ml photoinitiator (Irgacure 2959, Sigma Aldrich, USA) and 7.5µl PBS. The thiolated crosslinker was added at a 2:1 molar ratio of acrylate:thiol. The PEG-Ac solution was cast into a PDMS mold of 50µl hydrogel using UV irradiation at an intensity of 5mW/cm 2 (OmniCureR Series 1500, Excelitas Technologies Ltd, USA) for 5 min.
7
PEG hydrogel formation via UV photo-polymerization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PEG hydrogels using UV photo-polymerization PEG hydrogels were formed using free radical-based photo-polymerization, which is the most common method used to prepare biomimetic hydrogels [76] (link). To obtain 50µl of 5wt%. PEG-Ac, 5µl of 500mg/ml 4 arm-Ac-PEG (Mw = 10kDa, Laysan Bio, Inc., USA) were mixed with 2.5µl of 200 mg/ml protease degradable peptide crosslinker GCRDVPMSMRGGDRCG (Mw = 2kDa, Genscript, USA), 1µl of 5mg/ml photoinitiator (Irgacure 2959, Sigma Aldrich, USA) and 7.5µl PBS. The thiolated crosslinker was added at a 2:1 molar ratio of acrylate:thiol. The PEG-Ac solution was cast into a PDMS mold of 50µl hydrogel using UV irradiation at an intensity of 5mW/cm 2 (OmniCureR Series 1500, Excelitas Technologies Ltd, USA) for 5 min.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!