Goat pabs against rb igg hrp

Recombinant human transforming growth factor-β1 (TGF-β1) was purchased from Peprotech (United States). Antibodies against collagen Ⅰ, fibronectin, P-Smad3/Smad3, P-Akt/Akt, P-ERK/ERK, P-JNK/JNK, P-P38/P38, GAPDH, E-cadherin, vimentin, and N-cadherin were purchased from Cell Signaling Technology (United States). The β-tubulin antibody was obtained from Proteintech (China). The α-SMA antibody was obtained from Santa Cruz Biotechnology (China). Goat pAbs against Rb IgG (HRP) and Rb pAbs against Ms IgG were obtained from ImmunoWay (China). TRIzol reagent was acquired from Ambion Life Technology (China). DEPC-treated H₂O and RNase Away H₂O were purchased from Life Technologies. Fastking gDNA Dispelling RT SuperMix was purchased from Tiangen (Beijing, China). UNICON® qPCR SYBR Green Master Mix and liposomal transfection reagents were purchased from Yevsen (Shanghai, China). The Masson’s trichrome staining kit was obtained from Solarbio (China). Recombinant Mouse Platelet-derived Growth Factor-BB were purchased from MedChemExpress (China).

Recombinant human transforming growth factor‐β1 (TGF‐β1) was purchased from Peprotech. Antibodies against fibronectin, P‐mTOR/mTOR, P62, LC3I/II were purchased from Cell Signalling Technology. Antibodies against α‐SMA, P‐PI3K/PI3K, P‐Akt/Akt, GAPDH were obtained from Affinity Biosciences. Goat pAbs against Rb IgG (HRP) and Rb pAbs against Ms IgG were obtained from ImmunoWay (China). TRIzol reagent was acquired from Ambion Life Technology (China). DEPC‐treated H2O and RNAse Away H₂O were purchased from Life Technologies. Fastking gDNA Dispelling RT SuperMix was purchased from Tiangen. UNICON® qPCR SYBR Green Master Mix and liposomal transfection reagents were purchased from Yevsen. The Masson's trichrome staining kit was obtained from Solarbio (China).