Red seaweed G. fisheri was harvested in October 2020 from the Shrimp Genetic Improvement Center, Surat Thani, Thailand. Human kidney cell line (HK-2) was purchased from ATCC (Manassas, VA, USA). Dulbecco’s modified eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco, Life Technologies (Grand Island, NY, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution was purchased from PanReac AppliChem, Molecular Probes Inc. (Darmstadt, Germany). 1,1-Diphenyl-2-picrylhydrazyl (DPPH), dihydronicotine amide adenine dinucleotide (NADH), phenazine methosulfate (PMS), nitro blue tetrazolium (NBT), vitamin C, DEAE-sepharose fast flow, dextran standards (DS), phenylmethylsulfonyl fluoride (PMSF), protease inhibitor cocktail, sodium metaperiodate, sodium borohydride, sulphur trioxide-triethylamine complex, N,N-dimethylformamide, and deuterium oxide (D2O) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A Clarity™ Western ECL Substrate was purchased from the Bio-Rad Laboratories, Inc. (Hercules, CA, USA). Anti-caspase-3, anti-BCL-2, anti-β-actin, and HRP-conjugated goat anti-mouse IgG antibodies were purchased from the Cell Signaling Technology, Inc. (Danvers, MA, USA). Cystone was purchased from the Himaraya drug company (Bangalore, India). All other chemicals were purchased from Merck, Thailand.
Deae sepharose fast flow
Manufactured by Merck Group
Sourced in United States
DEAE-sepharose fast flow is a laboratory chromatography resin used for the purification and separation of proteins, nucleic acids, and other biomolecules. It consists of diethylaminoethyl (DEAE) functional groups coupled to a highly cross-linked agarose matrix, providing a strong anion-exchange medium with a fast flow rate.
Automatically generated - may contain errors
Lab products found in correlation
Pd 10 sephadex g 25m, by Merck Group (2 mentions)
Econo column, by Merck Group (2 mentions)
Spectra chrom lc, by Merck Group (2 mentions)
Amicon stirred cell concentrators, by Merck Group (2 mentions)
Sodium metaperiodate, by Merck Group (1 mentions)
Dextran standard, by Merck Group (1 mentions)
Nitroblue tetrazolium, by Merck Group (1 mentions)
Phenazine methosulfate, by Merck Group (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Hrp conjugated goat anti mouse igg antibody, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions)
Deuterium oxide d2o, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
Calcium chloride dihydrate, by Merck Group (1 mentions)
Mercaptoethanol, by Merck Group (1 mentions)
4 protocols using deae sepharose fast flow
1
Anticancer Potential of G. fisheri
2
Purification and Characterization of Cod Trypsin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dimethyl sulfoxide (DMSO), Succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide (Suc-AAPF-pNA), Benzoyl-L-Arg-p-nitroanilide (BAPNA), EDTA, Bis-Tris, glycine, sodium dodecyl sulphate (SDS), dithiothreitol (DTT), benzamidine, -mercaptoethanol, phenyl methyl sulphonyl fluoride (PMSF), soybean trypsin inhibitor, tosyl-lysyl-chloromethyl ketone (TLCK), Bowman-Birk trypsin inhibitor, 2-Amino-2-(hydroxymethyl)-1,3propanediol (TRIS), DEAE-Sepharose Fast Flow and Benzyloxycarbonyl-Gly-Pro-Arg pnitroanilide acetate salt (CBZ-GPR-pNA) were from Sigma Aldrich, St. Louis, MO, USA.
Calcium chloride dihydrate, sodium chloride, sodium hydroxide, methanol, glycerol, acetic acid and hydrochloric acid were from Merck, Darmstadt. Succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide (Suc-AAPA-pNA), Succinyl-L-Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide (Suc-AAPL-pNA) and Benzyloxycarbonyl-L-Phe-L-Arg-p-nitroanilide (CBZ-FR-pNA) were from Bachem AG, Bubendorf, Switzerland. Bio-Rad protein assay was from Bio-Rad laboratories, Hercules, California, USA. PageRuler Prestained Protein Ladder SDS from Fermentas (now Thermo Fisher Scientific, Rockford, IL). NucleoSpin Plasmid kit from Macherey-Nagel (US).
benzamidine purified cod trypsin was purified as described before [4] and obtained from Zymetech (Reykjavik, Iceland).
Calcium chloride dihydrate, sodium chloride, sodium hydroxide, methanol, glycerol, acetic acid and hydrochloric acid were from Merck, Darmstadt. Succinyl-L-Ala-L-Ala-L-Pro-L-Ala-p-nitroanilide (Suc-AAPA-pNA), Succinyl-L-Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide (Suc-AAPL-pNA) and Benzyloxycarbonyl-L-Phe-L-Arg-p-nitroanilide (CBZ-FR-pNA) were from Bachem AG, Bubendorf, Switzerland. Bio-Rad protein assay was from Bio-Rad laboratories, Hercules, California, USA. PageRuler Prestained Protein Ladder SDS from Fermentas (now Thermo Fisher Scientific, Rockford, IL). NucleoSpin Plasmid kit from Macherey-Nagel (US).
benzamidine purified cod trypsin was purified as described before [4] and obtained from Zymetech (Reykjavik, Iceland).
3
Synthesis and Purification of 2-Oxo-3-pentynoate
4
Protein Purification Techniques
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