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Cy3 labeled linc00839 probe

Manufactured by RiboBio
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Sourced in China

The Cy3-labeled LINC00839 probe is a laboratory tool used to detect and visualize the expression of the LINC00839 gene in biological samples. It consists of a Cy3 fluorescent dye molecule attached to a nucleic acid sequence that is complementary to the LINC00839 transcript. The probe can be used in various molecular biology techniques, such as in situ hybridization, to provide a reliable and sensitive method for analyzing the presence and distribution of LINC00839 in cells or tissues.

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Lab products found in correlation

Lsm980 confocal laser scanning microscope, by Zeiss (1 mentions) Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions) Fluorescence microscopy, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Fish kit, by RiboBio (1 mentions)

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Cy3-labeled probes

2 protocols using cy3 labeled linc00839 probe

1

Nuclear-Cytoplasmic RNA Extraction and FISH Analysis

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Nuclear and cytoplasmic RNA was separated with the NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen) and then analyzed by RT–qPCR. For FISH assays, cells were first grown for 24 h in 24-well plates with glass cover slips. After immobilization and permeabilization, cells were hybridized with 20 μM Cy3-labeled LINC00839 probe (RiboBio), and 6-diamidino-2-phenylindole was used to stain nuclei. Images were observed with an LSM 980 confocal laser-scanning microscope (ZEISS).
Zheng W.H., Long Z.Q., Zheng Z.Q., Zhang L.L., Liang Y.L., Li Z.X., Lv J.W., Kou J., Hong X.H., He S.W., Xu R., Zhou G.Q., Liu N., Ma J., Sun Y., Lin L, & Wei D. (2023). m6A-enriched lncRNA LINC00839 promotes tumor progression by enhancing TAF15-mediated transcription of amine oxidase AOC1 in nasopharyngeal carcinoma. The Journal of Biological Chemistry, 299(7), 104873.
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2

Fluorescent In Situ Hybridization Assay

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FAM-labeled miR-454-3p probe and Cy3-labeled LINC00839 probe were obtained from RiboBio (Guangzhou, China). FISH assay was performed using a FISH kit (RiboBio) according to the manufacturer’s protocol. In brief, the probes were hybridized overnight. Then, cell nuclei were counterstained using DAPI (Beyotime). Fluorescence was measured using a fluorescence microscopy (Carl Zeiss, Germany).
Zhang F.Y., Li X., Huang T.T., Xiang M.L., Sun L.L, & Sun Z.L. (2021). LINC00839 knockdown restrains the metastatic behavior of nasopharyngeal carcinoma by sponging miR-454-3p. Aging (Albany NY), 13(24), 26022-26033.
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