Lb medium

Manufactured by Hopebio

Sourced in China

LB medium is a widely used nutrient-rich growth medium for culturing bacteria. It provides essential nutrients and supports the growth of a variety of bacterial species. The medium consists of a combination of tryptone, yeast extract, and sodium chloride, which together create an optimal environment for bacterial proliferation.

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Lab products found in correlation

Tsb ye, by Hopebio (1 mentions) E coli bl21 de3, by Sangon (1 mentions) E coli dh5α, by Sangon (1 mentions)

5 protocols using lb medium

Uranium Biosorption by Bacillus megaterium

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Experimental reagents: sodium alginate (Shanghai Titan Technology Co., Ltd, China), U₃O₈ (Xi'an Dingtian Chemical Co., Ltd, China), hydrochloric acid and nitric acid (Guiyang Yida Chemical Reagent Co., Ltd, China), LB medium (Qingdao Hope Biotechnology Co., Ltd), potassium dihydrogen phosphate, potassium hydrogen phosphate and calcium chloride (Tianjin Kaitong Chemical Reagent Co., Ltd, China, analytical grade).
Strain: B. megaterium (China General Microbiological Culture Collection Center, No.: 1.10466).

Li D., Yang Y., Zhang P., Liu J., Li T, & Yang J. (2021). U(VI) adsorption in water by sodium alginate modified Bacillus megaterium. Royal Society Open Science, 8(2), 202098.

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Microbial Growth Modulation with miRNA and Ginsenoside

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B. vulgatus (ATCC 8428, Microbiologics, USA) and Escherichia coli (E. coli, provided by Wei Fenghuan research group of Southern Medical University) were cultured respectively in Difco-Reinforced Clostridial Medium (BD Bioscience, New Jersey, USA) and LB medium (Hopebio, Shandong, China) supplemented with miRNA mimics, scramble miRNAs, inhibitor miRNAs and ginsenoside Rg1 at 37°C and monitored as absorbance at 600 nm (OD600).

Li C., Lin Y., Lin R., Chen Z., Zhou Q., Luo C, & Mo Z. (2023). Host miR-129-5p reverses effects of ginsenoside Rg1 on morphine reward possibly mediated by changes in B. vulgatus and serotonin metabolism in hippocampus. Gut Microbes, 15(2), 2254946.

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Bacterial Strains and Culture Conditions

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The bacterial strains used in this study are described in Table 1. These include a broad range of reference strains as well as multidrug-resistant strains. All strains were stored frozen at −80 °C in 20% (vol/vol) glycerol and cultured in LB medium at 37 °C. Vector pET-28b, E. coli BL21, and E. coli C41 were prepared for cloning and protein expression (Novagen, USA). TSB-YE and LB medium were purchased from Hopebio (Qingdao, China). The protein markers were purchased from SMOBIO (Hsinchu City, Taiwan).

Ding Y., Zhang Y., Huang C., Wang J, & Wang X. (2020). An Endolysin LysSE24 by Bacteriophage LPSE1 Confers Specific Bactericidal Activity against Multidrug-Resistant Salmonella Strains. Microorganisms, 8(5), 737.

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Isolation and Cultivation of Marine Bacterium

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N. mentallicus SCSIO 53858 (Genbank No. SUB14163607) was isolated from a deep-sea sediment sample collected from the South China Sea (118°48′01″ E, 18°17′37″ N) at a depth of 4000 m. The strain was deposited at the RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences. Each isolate was cultured in marine broth 2216 (MB, BD Difco).
The bacterial strain C. violaceum CV026 was purchased from the Guangdong Provincial Center for Microbial Strains (Guangzhou, China). The bacterial strain was stored at −80 °C in a refrigerator until use. Each isolate was cultured in LB medium (Hopebio).

Li Y., Ding W., Yin J., Li X., Tian X., Xiao Z., Wang F, & Yin H. (2024). 2,3-Dimethoxycinnamic Acid from a Marine Actinomycete, a Promising Quorum Sensing Inhibitor in Chromobacterium violaceum. Marine Drugs, 22(4), 177.

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Culturing and Characterizing Bacterial Strains

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V. parahaemolyticus 17802, Salmonella typhimurium 14028 and Staphylococcus aureus 43300 were purchased from the American Type Culture Collection (ATCC). V. parahaemolyticus isolates VP 1.1, VP 1.3, VP 2.2, VP 3.1, VP 3.2, VP 4.1, VP 304, VP 461, VP 800 and VP l4-90, Vibrio harveyi V1, Shewanella baltica S1, Acinetobacter baumannii A1, Pseudomonas fluorescens P1, Aeromonas hydrophila A1, Pseudomonas putrefaciens P1, Vibrio anguillarum V1 were stored in the Food Safety Lab at the Ocean University of China. E. coli DH5α and E. coli BL21 (DE3) were purchased from Sangon Biotech (Shanghai, China). All marine bacterial species were routinely cultured in the Zobell 2216 marine medium (Hopebio, Qingdao, China), whereas the other bacteria were grown in LB medium (Hopebio, Qingdao, China). Working concentrations of antibiotics were added into the growth medium when necessary.

Wang L., Ju X., Cong Y., Lin H, & Wang J. (2022). A Single Catalytic Endolysin Domain Plychap001: Characterization and Application to Control Vibrio parahaemolyticus and Its Biofilm Directly. Foods, 11(11), 1578.

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