Anti β actin hrp a3854

Manufactured by Merck Group

Sourced in United States

Anti-β-actin-HRP (A3854) is a laboratory reagent produced by Merck Group. It is an antibody conjugated with horseradish peroxidase (HRP) that binds to the β-actin protein. This product can be used for detection and quantification of β-actin in various research applications.

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Lab products found in correlation

Close spelling variants of this product

β-actin (6886 citations) Anti-β-actin (3256 citations) Anti-actin (1282 citations) A3854 (103 citations) Anti-β-actin-HRP (43 citations) Actin-HRP (20 citations) β-actin-HRP (18 citations) Anti-β-ACTIN (A3854) (13 citations) Anti-actin-HRP (12 citations) β-actin (A3854) (5 citations)

2 protocols using anti β actin hrp a3854

Biochemical Assays for Inflammation

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Aβ was purchased by Biochem (Germany). Atropine sulphate, SP, guanethidine monosulphate, nigericin, Ac-YVAD-cmk, bacterial LPS (Escherichia coli 026:B6) and DMSO, sucrose, Tris, EGTA, Triton X-100, Tris-buffer, DTNB, acetil-coenzyme A, oxaloacetate and anti-β-actin-HRP (A3854) were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Foetal bovine serum (FBS) was obtained from PAA Laboratories. TTX, GR159897, SB218795, L-NAME were obtained from Tocris (Bristol, UK). Anti-IL-1β (anti-human) was purchased by R&D, rabbit anti-ASC (SC-22514-R) by Santa Cruz. Secondary antibody HRP conjugates were from DAKO. Alexa Fluor 594-conjugated donkey anti-rabbit antibody (A-21207) was purchased by Invitrogen.

Pellegrini C., Daniele S., Antonioli L., Benvenuti L., D’Antongiovanni V., Piccarducci R., Pietrobono D., Citi V., Piragine E., Flori L., Ippolito C., Segnani C., Palazon-Riquelme P., Lopez-Castejon G., Martelli A., Colucci R., Bernardini N., Trincavelli M.L., Calderone V., Martini C., Blandizzi C, & Fornai M. (2020). Prodromal Intestinal Events in Alzheimer’s Disease (AD): Colonic Dysmotility and Inflammation Are Associated with Enteric AD-Related Protein Deposition. International Journal of Molecular Sciences, 21(10), 3523.

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Evaluating SIRT1, CTSS, and NF-κB Signaling

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Anti-SIRT1 (sc-15404), anti-CTSS (sc-6505), anti-p65-NFκB (sc-372), anti-p50-NFκB (sc-7178), anti-Lamin (sc-6215) were taken from Santa Cruz Biotechnology; anti-β-actin-HRP (A3854) from Sigma-Aldrich; anti-alpha smooth muscle actin (A5228) from Sigma; and anti-CTSB (06-480) from Millipore (Billerica, MA, USA).

de Mingo Á., de Gregorio E., Moles A., Tarrats N., Tutusaus A., Colell A., Fernandez-Checa J.C., Morales A, & Marí M. (2016). Cysteine cathepsins control hepatic NF-κB-dependent inflammation via sirtuin-1 regulation. Cell Death & Disease, 7(11), e2464-.

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