Ab17732

For western blot analysis, cells were lysed with the CelLytic^™ Cell Lysis Reagent (Sigma, Saint Louis, MO, USA) supplemented with a protease inhibitor ‘cocktail’, the protein concentrations in the extracts were measured using the bicinchoninic acid assay (Pierce, Rockford, IL, USA), and then, the volumes were made equal using the extraction reagent. Equal amounts of extracts were separated by SDS/PAGE, and then, they were transferred onto polyvinylidene fluoride membranes for immunoblot analysis as described previously 12, 13. Rabbit polyclonal antibody to SNAI1 (ab‐17732; Abcam, Cambridge, MA, USA), mouse mAb to Cyclin D1 (ab6152; Abcam, Cambridge, MA, USA), mouse mAb to Cyclin A (4656; Cell Signaling Technology, Boston, MA, USA), rabbit mAb to Bcl‐2 (2870; Cell Signaling Technology), rabbit mAb to cytochrome c (4280; Cell Signaling Technology), mouse mAb to anti‐Caspase‐3 (9668; Cell Signaling Technology), mouse mAb to N‐Cadherin (14215; Cell Signaling Technology), rabbit mAb to E‐Cadherin (3195; Cell Signaling Technology), mouse mAb to β‐actin (TA‐09; ZSGB‐BIO, Beijing, China), and the corresponding HRP‐conjugated secondary antibody (sc‐2004 and sc‐2005; Santa Cruz Biotechnology, Dallas, TX, USA) were used for immunoblot analysis.