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24 well plate

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The 24-well plates are a laboratory equipment used for cell culture and various assays. They provide 24 individual wells, allowing for the simultaneous cultivation or testing of multiple samples or conditions in a single plate.

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Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (4 mentions) Acti stain 555, by Cytoskeleton (2 mentions) Plan fluor lens, by Nikon (2 mentions) Ocar er, by Nikon (2 mentions) Triton x 100, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Prolong antifade, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) 35 mm glass bottomed plastic dishes, by MatTek (1 mentions) Matrigel coated, by BD (1 mentions) Las af, by Leica (1 mentions) Ax10 imager a2 microscope, by Zeiss (1 mentions) Fluorophore conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

24-well glass-bottomedcell culture plates No. 1.5 glass-bottom 24 well plates Gelatin coated glass bottom 24 well plates Glass-bottom 24-well plates Glass-bottomed 24 well plate

6 protocols using 24 well plate

1

Immunofluorescence Staining Protocol

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Cells were grown on coverslips or glassbottom dishes (24-well plates; MatTek), washed twice with PBS, and fixed for 10 min in PHEM buffer (20 mM PIPES pH 6.8, 0.2% Triton X-100, 10 mM EGTA, and 1 mM MgCl2) containing 4% paraformaldehyde. For immunofluorescence staining, coverslips were washed twice with PBS, and then blocked 100% goat serum albumin for 1 h before the indicated primary antibody was applied. Cells were then washed with PBS and incubated with Alexa Fluor 488-, 555-, or 647-conjugated secondary antibody (Molecular Probes). Cell nuclei were stained with DAPI (Sigma). After washing, coverslips were mounted onto glass microscope slides with Prolong antifade (Invitrogen).
d’Alcontres M.S., Palacios J.A., Mejias D, & Blasco M.A. (2014). TopoIIα prevents telomere fragility and formation of ultra thin DNA bridges during mitosis through TRF1-dependent binding to telomeres. Cell Cycle, 13(9), 1463-1481.
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2

Culturing GLUTag cells for peptide secretion

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GLUTag cells, an established pro-glucagon-derived peptide-secreting cell line model [23 (link)], were cultured as previously described [24 (link)]. Cells for experimental use were plated onto 1% Matrigel-coated (BD BioScience) 24-well plates (secretion) or 35 mm glass-bottomed plastic dishes (MatTek Corporation, Ashland, MA, USA) (imaging). Secretion experiments were carried out 18-24 h after plating. Imaging experiments were carried out 18-48 h after plating.
Psichas A., Larraufie P.F., Goldspink D.A., Gribble F.M, & Reimann F. (2017). Chylomicrons stimulate incretin secretion in mouse and human cells. Diabetologia, 60(12), 2475-2485.
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3

Time-Lapse Videomicroscopy of Mitotic Progression

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For time-lapse videomicroscopy, MEFs of the indicated genotypes expressing Histone H2B-Cherry were plated on glassbottom dishes (24 well plates; MatTek) and transfected with siGenome SMART pool against Mad2 (Thermo Scientific Dharmacon), mouse MAD2L1 M-059314-01-0005) where indicated. After 24 h, cells were followed by time-lapse microscopy in fresh medium or in the presence of nocodazole for 48 h. Image acquisition was performed using a CCD fluorescence microscope (Leica) equipped with a selective filter for Cherry emission. Images were acquired at 10 and 4 min intervals using LAS AF (Leica) software for analysis.
d’Alcontres M.S., Palacios J.A., Mejias D, & Blasco M.A. (2014). TopoIIα prevents telomere fragility and formation of ultra thin DNA bridges during mitosis through TRF1-dependent binding to telomeres. Cell Cycle, 13(9), 1463-1481.
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4

Fluorescence Microscopy Imaging of Cell Morphology

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Approximately 12,000 cells were plated in each well of a 24-well plate (MatTek, Ashland, MA, USA). After 24 h incubation, cells were fixed with 4% para-formaldehyde, permeabilized with Triton X-100 (Sigma-Aldrich), and blocked for nonspecific binding with 5% goat serum. Nuclear DNA was stained with DAPI, and actin was stained with either phalloidin Alexa Fluor 488 (Thermo Fisher Scientific) or Acti-stain 555 (Cytoskeleton Inc., Denver, CO, USA). For each sample, fluorescently labeled cells in seventy-two (8-by-9 square grid) fields of view from a low-magnification lens (10× Plan Fluor lens; N.A. 0.3, Nikon) with a CCD (Hamamatsu OCAR-ER) on a Nikon NI microscope covering a contiguous area of approximately 6.0 mm × 5.0 mm (30.0 mm2) were analyzed. DAPI (nucleus) or phalloidin (F-actin) fluorescence was recorded to obtain morphometric information about the nucleus and cellular body of each cell within the scanning region. Segmentation of nuclear and cellular shape from images was conducted using a custom MATLAB code. At least 500 – 2500 cells/sample were analyzed for statistical analysis.
Yu Y., Rahmanto Y.S., Lee M.H., Wu P.H., Phillip J.M., Huang C.H., Vitolo M.I., Gaillard S., Martin S.S., Wirtz D., Shih I.M, & Wang T.L. (2018). Inhibition of ovarian tumor cell invasiveness by targeting SYK in tyrosine kinase signaling pathway. Oncogene, 37(28), 3778-3789.
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5

Immunostaining of Cultured Cells

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Cells were cultured on coverslips in a 24-well plate (MatTek, Ashland, MA). For immunostaining, the cells were fixed in 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, and blocked with 5% BSA for 1 h. After that, cells were incubated with primary antibodies (100 times dilution) for 1 h and washed three times with PBST (PBS with 0.1% Tween-20). After incubation with appropriate fluorophore-conjugated secondary antibodies (Molecular Probes) and DAPI (Thermo Fisher), the coverslips were mounted on slides. The pictures were photographed with AX10 imager A2 microscope (Carl Zeiss MicroImaging).
Yuan K., Lan J., Xu L., Feng X., Liao H., Xie K., Wu H, & Zeng Y. (2022). Long noncoding RNA TLNC1 promotes the growth and metastasis of liver cancer via inhibition of p53 signaling. Molecular Cancer, 21, 105.
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6

Fluorescence Microscopy Imaging of Cell Morphology

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Approximately 12,000 cells were plated in each well of a 24-well plate (MatTek, Ashland, MA, USA). After 24 h incubation, cells were fixed with 4% para-formaldehyde, permeabilized with Triton X-100 (Sigma-Aldrich), and blocked for nonspecific binding with 5% goat serum. Nuclear DNA was stained with DAPI, and actin was stained with either phalloidin Alexa Fluor 488 (Thermo Fisher Scientific) or Acti-stain 555 (Cytoskeleton Inc., Denver, CO, USA). For each sample, fluorescently labeled cells in seventy-two (8-by-9 square grid) fields of view from a low-magnification lens (10× Plan Fluor lens; N.A. 0.3, Nikon) with a CCD (Hamamatsu OCAR-ER) on a Nikon NI microscope covering a contiguous area of approximately 6.0 mm × 5.0 mm (30.0 mm2) were analyzed. DAPI (nucleus) or phalloidin (F-actin) fluorescence was recorded to obtain morphometric information about the nucleus and cellular body of each cell within the scanning region. Segmentation of nuclear and cellular shape from images was conducted using a custom MATLAB code. At least 500 – 2500 cells/sample were analyzed for statistical analysis.
Yu Y., Rahmanto Y.S., Lee M.H., Wu P.H., Phillip J.M., Huang C.H., Vitolo M.I., Gaillard S., Martin S.S., Wirtz D., Shih I.M, & Wang T.L. (2018). Inhibition of ovarian tumor cell invasiveness by targeting SYK in tyrosine kinase signaling pathway. Oncogene, 37(28), 3778-3789.
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