Human gastric cancer cell lines MGC-803 was brought from the Shanghai Genechem Co., Ltd. (GENE, Shanghai, China) and SGC-7901 was purchased from the Shanghai Biowing Applied Biotechnology Co., Ltd. (BIOWING, Shanghai, China). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY). When the cell density reached 70%, the cells were treated with 2 μM, 10 μM, or 20 μM ISO (HARVEST, Shanghai, China) or 20 μM, 40 μM, or 50 μM ICI118,551 (a specific β2-AR antagonist) (Abcam, #ab120808, US), while the control group was given matching normal saline. MGC-803 cells and SGC-7901 cells were harvested and prepared for the next trial after 12 hours and 24 hours, respectively.
Ici118 551
Manufactured by Abcam
Sourced in China, United Kingdom
ICI118,551 is a highly selective and potent beta-2 adrenergic receptor antagonist. It is used in scientific research to study the role of beta-2 adrenergic receptors in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Mgc 803, by Genechem (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rabbit igg isotype control, by GeneTex (1 mentions)
Anti tyrosine hydroxylase, by Santa Cruz Biotechnology (1 mentions)
Anti mmp2, by Abcam (1 mentions)
Anti slug antibody, by Cell Signaling Technology (1 mentions)
Anti mmp9, by Abcam (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Phentolamine hydrochloride, by Merck Group (1 mentions)
Anti e cadherin, by GeneTex (1 mentions)
Anti β2 ar, by Abcam (1 mentions)
2 protocols using ici118 551
1
Exploring ISO and ICI118,551 Effects on Gastric Cancer Cells
2
Adrenergic Receptor Regulation in Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-tyrosine hydroxylase (TH) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-β2-AR, anti-MMP2, anti-MMP9, anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase) antibodies, and β2-AR antagonist ICI118,551 were purchased from Abcam (Cambridge, UK). Anti-α1B-AR and anti-α2A-AR antibodies were purchased from Proteintech Group (Wuhan, China). Anti-Slug antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-E-cadherin, anti-β1-AR, anti-vimentin antibodies, and rabbit IgG isotype control were purchased from GeneTex (San Antonio, TX, USA). Adrenergic receptor agonist NE was purchased from Aladdin (Shanghai, China). Nonselective α-AR blocker phentolamine hydrochloride was purchased from Sigma-Aldrich (Saint Louis, MO, USA).
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