Terminal deoxynucleotidyl transferase mediated dutp nick end labeling tunel kit

Picroside II (purity >99%) was purchased from the Chinese National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). 2,3,5-triphenyltetrazolium chloride (TTC) was obtained from Sigma-Aldrich (St. Louis, MO, USA). The creatine kinase (CK) and lactate dehydrogenase (LDH) assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) kit was from Roche (Indianapolis, IN, USA). The antibodies targeting HMGB1, RAGE, TLR-2, TLR-4 and β-actin were purchased from Abcam (Cambridge, UK). TRIzol reagent was a product of Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The First Strand cDNA Synthesis kit was obtained from MBI Fermentas, Inc. (Vilnius, Lithuania) and the ELISA kits of TNF-α, IL-6, IL-1β, intercellular adhesion molecule-1 (ICAM-1), HMGB1 and NF-κB p65 were purchased from Shanghai Jiang Lai Biotechnology Co., Ltd. (Shanghai, China).

The human osteoblast cell line HCO was purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. α-MEM culture solution and fetal bovine serum (FBS) were purchased from Hyclone, USA. Trypsin, penicillin-streptomycin and phosphate buffered saline (PBS) were purchased from Solarbio, China. Cell culture flask and other consumables were purchased from NEST, USA. Serum-free Opti medium and TRIzol reagent were purchased from Invitrogen, USA. Methyl thiazolyl tetrazolium (MTT) powder was purchased from Biosharp, China. Organic reagents, such as dimethyl sulfoxide (DMSO), were purchased from Shanghai Rhawn Technology Development Co., Ltd. The reverse transcription kit, transfection reagent X-treme, RNA Keeper and SYBR Green reagent were purchased from Vazyme, China. MiR-214 mimics, miR-214 AMO and its negative control (NC) and PCR primers were designed and synthesized by Shanghai Generay Biotechnology Co., Ltd. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) kit was bought from Roche, Switzerland. 4% paraformaldehyde, protein lysis buffer, Hoechst staining solution, BCA protein concentration assay kit and protein loading buffer were bought from Beyotime, China.