Amersham ecl primer

Manufactured by GE Healthcare

The Amersham ECL Primer is a lab equipment product designed for the detection of proteins using enhanced chemiluminescence (ECL) technology. It provides a sensitive and reliable method for protein analysis.

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Lab products found in correlation

Irdye 800cw, by LI COR (2 mentions) Infrared scanner, by LI COR (2 mentions) Amersham ecl, by Cytiva (2 mentions)

Close spelling variants of this product

Amersham ECL (119 citations) AmershamTM ECLTM (3 citations) ECL primer (3 citations) Amersham ECLTM Primer Western Blotting Detection Reagent (2 citations) AMERSHAM ECL Primer Western Blotting Detection Reagent (2 citations)

2 protocols using amersham ecl primer

Co-Immunoprecipitation and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

For co-immunoprecipitation, eluted products and 10% of the input were separated by SDS-PAGE and transferred to a PVDF membrane for blotting at 4C with Gli3 (polyclonal goat IgG 1:1000, R and D Systems) and Hand2 (polyclonal goat IgG or mouse monoclonal IgG₁ 1:1000) primary antibodies. Detection of primary antibodies was performed using infrared-conjugated secondary antibodies (donkey anti-goat or goat anti-mouse IRDye 800CW, LICOR) and acquired using a LICOR infrared scanner. For plasmid verification, F primary antibody (monoclonal M2 mouse IgG₁) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.

Elliott K.H., Chen X., Salomone J., Chaturvedi P., Schultz P.A., Balchand S.K., Servetas J.D., Zuniga A., Zeller R., Gebelein B., Weirauch M.T., Peterson K.A, & Brugmann S.A. (2020). Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks. eLife, 9, e56450.

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Co-immunoprecipitation and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

For co-immunoprecipitation, eluted products and 10% of the input were separated by SDS-PAGE and transferred to a PVDF membrane for blotting at 4C with Gli3 (polyclonal goat IgG 1:1000, R&D Systems) and Hand2 (polyclonal goat IgG or mouse monoclonal IgG1 1:1000) primary antibodies. Detection of primary antibodies was performed using infrared-conjugated secondary antibodies (donkey anti-goat or goat antimouse IRDye 800CW, LICOR) and acquired using a LICOR infrared scanner. For plasmid verification, Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.

Elliott K.H., Chen X., Salomone J., Chaturvedi P., Schultz P.A., Balchand S.K., Servetas J.D., Zúñiga A., Zeller R., Gebelein B., Weirauch M.T., Peterson K.A., & Brugmann S.A. (2020). Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks.

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