Ly294002 hydrochloride

Manufactured by Bio-Techne

Sourced in United Kingdom

LY294002 hydrochloride is a laboratory reagent produced by Bio-Techne. It is a specific and potent inhibitor of phosphoinositide 3-kinase (PI3K). The compound can be used for in vitro research purposes.

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Lab products found in correlation

Celltitre 96 aqueous one solution cell proliferation assay, by Promega (1 mentions) Pd98059, by Bio-Techne (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Gant61, by Selleck Chemicals (1 mentions) Hpi 1, by Bio-Techne (1 mentions) Vismodegib gdc 0449, by Selleck Chemicals (1 mentions) U0126, by Bio-Techne (1 mentions) Sirtinol, by Enzo Life Sciences (1 mentions) Fk866, by Adipogen (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

3 protocols using ly294002 hydrochloride

Cell Proliferation Assay for HAECs and HASMCs

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Cell Titre 96 AQueous One Solution Cell Proliferation Assay (Catalog No. G3581; Promega, Southampton, UK) was performed according to manufacturer's instructions in HAECs and HASMCs. Cells were seeded at approximately 5,000 cells per well in 96‐well plates and left to adhere for at least 4 hr. Cells were then treated with control media, or media supplemented with 0.1 ng/ml, 0.5 ng/ml, 1 ng/ml, 10 ng/ml or 50 ng/ml of ucOCN for 48 or 72 hr. The proliferation assay was repeated with 10 ng/ml of ucOCN on confluent cells to establish if the effects of OCN were due to an increase in cell proliferation as opposed to an increase in cell metabolism.
The proliferation assay was repeated using 10 ng/ml of ucOCN alone or in the presence of 3 µM PI3K inhibitor (LY294002 hydrochloride; Catalog No. 1130; Tocris Bioscience, Bristol, UK) or MAPK (MKK/MEK) inhibitor (PD98059; Catalog No. 1213; Tocris Bioscience).

Millar S.A., Anderson S.I, & O'sullivan S.E. (2019). Human vascular cell responses to the circulating bone hormone osteocalcin. Journal of Cellular Physiology, 234(11), 21039-21048.

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Evaluation of Hedgehog Pathway Inhibitors

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The tyrosine kinase inhibitor (TKI) dasatinib, SMO agonist (SAG)- hydrochloride, GLI1/2 inhibitor GANT61, and SMO antagonist vismodegib (GDC-0449) were purchased from Selleckchem (Houston, TX). GLI1/2 inhibitor HPI-1, PI3Kα/δ/β inhibitor LY-294002 hydrochloride, and MEK1/2 inhibitor U0126 were purchased from Tocris (Minneapolis, MN). High activity recombinant Human Sonic Hedgehog (SHH) Protein was purchased from R&D Systems (Minneapolis, MN) and arsenic trioxide (A₂O₃ or ATO) from Millipore Sigma (Burlington, MA).

Falduto G.H., Pfeiffer A., Zhang Q., Yin Y., Metcalfe D.D, & Olivera A. (2022). A Critical Function for the Transcription Factors GLI1 and GLI2 in the Proliferation and Survival of Human Mast Cells. Frontiers in Immunology, 13, 841045.

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Metabolic Regulation in Cancer Cells

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HT29, COLO 205 and COLO 741 cells were provided by Roland Rad (TU Munich). BRAF-mutant COLO 741 were initially classified as colorectal cancer cells, but are now considered to derive from a pelvic melanoma metastasis [24] (link). Therefore, data obtained with COLO 741 are not shown except for immunoblots. HT29 cells were maintained in DMEM (high glucose), RKO cells (kindly provided by Bert Vogelstein) were maintained in MyCoy's 5A modified medium, COLO 741 and COLO 205 cells were kept in RPMI 1640 medium. All media were supplemented with 10% FCS, 1% L-glutamine and 1% penicillin/streptomycin (Invitrogen). FK866 (AdipoGen), sirtinol (Enzo Life Sciences) and the PI3K inhibitor (LY294002 hydrochloride (Tocris)) were dissolved in DMSO and stored at −20 °C. Cells were seeded at a concentration of 0.5 × 10⁴ in 12 well plates. Thirty hours after seeding, FK866, the solvent DMSO, or FK866 combined with LY294002 were added to sub-confluent cells for 72 hours. sirtinol, the solvent DMSO, or sirtinol combined with LY294002 were added for 48 hours.

Brandl L., Zhang Y., Kirstein N., Sendelhofert A., Boos S.L., Jung P., Greten F., Rad R, & Menssen A. (2019). Targeting c-MYC through Interference with NAMPT and SIRT1 and Their Association to Oncogenic Drivers in Murine Serrated Intestinal Tumorigenesis. Neoplasia (New York, N.Y.), 21(10), 974-988.

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