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Real time turbidimeter la 320c

Manufactured by Eiken Chemical
Sourced in Japan

The Real-time turbidimeter LA-320C is a laboratory instrument designed to measure the turbidity of liquid samples. It provides real-time measurements of the degree of light scattered by suspended particles in the sample, enabling the assessment of its clarity or cloudiness.

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5 protocols using real time turbidimeter la 320c

1

Isothermal CRISPR-Cas12a Nucleic Acid Extraction

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Genomic DNA kits for nucleic acid extraction and purification were purchased from Beijing TransGen Biotech. Co, Ltd (Beijing, China). The universal isothermal amplification kit was provided from HuiDeXin Biotech. Co., Ltd. (Tianjin, China). EnGen® Lba Cas12a (Cpf1) and 10 × NE Buffer 2.1 were purchased from New England Biolabs (MA, USA). The real-time turbidimeter (LA-320C) was purchased from Eiken Chemical. Co, Ltd (Japan). The ABI7500 real-time fluorescent quantitative PCR systems was purchased from Applied Biosystems (USA). The BluSight Pro (GD50502) was obtained from Manod Biotech. Co, Ltd (Suzhou, China).
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2

Rapid MPOX Diagnostic Assay Development

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A standard plasmid (ATI-plasmid), containing partial sequence of ATI gene(GenBank accession: MT903346.1) shared by both the Clade one (I) (the former Congo Basin (Central African) clade) and the Clade two (II) (the former West African clade) of the causative agent of MPOX, was constructed by Tianyi-Huiyuan Biotech Co., Ltd. (Beijing, China). A pseudotyped virus, which also contained the ATI gene, was constructed by Sangon Biotech. Co., Ltd. (Shanghai, China). Both common and labeled primers used in this study were synthesized by AOKE Biotech Co., Ltd. (Beijing, China). Visual indicator (VI) [used as visual detection regent (VDR)], DNA Isothermal Amplification Kit and LFB were all provided by HUIDEXIN Biotech Co., Ltd. (Tianjin, China). Genomic DNA kit for nucleic acid extraction and purification was purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). Real-time turbidimeter LA-320C was purchased from Eiken Chemical Co., Ltd., Japan.
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3

Isothermal Amplification with Visual Detection

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DNA Isothermal Amplification Kit, restriction endonuclease (Nb.BsrDI), visual detection reagent (VDR), biotin-dCTP, and nanoparticle-based lateral flow biosensor (LFB) were obtained from Huidexin Biotech Co., Ltd. (Tianjin, China). Primers and labeled primers used in this study were synthesized by AoKe Biotech Co., Ltd (Beijing, China). DNA extraction and purification kits were purchased from TransGen Biotech Co., Ltd (Beijing, China). Real-time turbidimeter LA-320C was purchased from Eiken Chemical Co., Ltd, Japan. Fluorescence quantitative PCR instrument ABI 7500 was purchased from Applied Biosystem Inc., USA.
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4

Nanoparticle-based Lateral Flow Biosensor

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Both common and labeled primers used in this study were synthesized by Aoke BiotechCo., Ltd. (Beijing, China). Visual detection reagent (VDR), biotin-14-dCTP, DNA Isothermal Amplification Kit, and nanoparticle-based lateral flow biosensor (LFB) were provided by Huidexin Biotech Co., Ltd. (Tianjin, China). Restriction endonuclease (Nb.BsrDI) was obtained from New England Biolabs Inc. (United States). Genomic DNA kit for nucleic acid extraction and purification was purchased from Beijing Transgen Biotech Co., Ltd. (Beijing, China). Real-time turbidimeter LA-320C was purchased from Eiken Chemical Co., Ltd. (Japan). The BlueSight Pro (GD50502) was purchased from Manod Biotech Co., Ltd. (Suzhou, China).
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5

Isothermal Amplification and Detection

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Both common and labeled primers used in this study were synthesized by AOKE Biotech Co., Ltd (Beijing, China). VDR, DNA Isothermal Amplification Kit, and LFB were all provided by HUIDEXIN Biotech Co., Ltd (Tianjin, China). Genomic DNA kit for nucleic acid extraction and purification was purchased from Beijing TransGen Biotech Co., Ltd (Beijing, China). Real-time PCR kit was purchased from Sansure BioTech Co., Ltd (Changsha, China) (http://www.sansure.com.cn/). Real-time turbidimeter LA-320C was purchased from Eiken Chemical Co., Ltd, Japan.
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