Ls180

The human colon adenocarcinoma cell lines, LS180 and Caco-2, were purchased from European Collection of Cell Cultures (ECACC) collections (KAC, Hyogo, Japan). LS180 cells were cultured in Dulbecco’s modified Eagle medium (DMEM) containing 1,500 mg/L glucose (FUJIFILM Wako Pure Chemical, Osaka, Japan) and Caco-2 cells were cultured in DMEM with 4,500 mg/L glucose. In both the cases, the medium was supplemented with heat-inactivated 10% fetal bovine serum (FBS) (Cosmo Bio, Tokyo, Japan). The cultures were maintained at 37°C in a humidified atmosphere with 5% CO₂.
The acidic cell culture medium (pH 6.5) was obtained by the addition of 35–37% HCl solution to DMEM containing 1,500 mg/L glucose supplemented with heat-inactivated 10% FBS by measuring pH with a FiveEasy Plus FEP20 pH Meter (METTLER TOLEDO, Tokyo, Japan). LS180 cells were grown for a sufficient time in normal medium and were subsequently maintained in the acidic cell culture medium (pH 6.5) for a few days as described previously with some modifications [26 (link)–28 (link)].

LS180 and HCT116 (colon cancer) cells were obtained from DS Pharma Biomedical (Osaka, Japan) and Dr. B. Vogelstein (Johns Hopkins University, Baltimore, MD), respectively.
LS180 cells were cultured in Eagle's Minimum Essential Medium (Wako, Osaka, Japan). HCT116 cells were maintained in Dulbecco's Modified Eagle's Medium (Wako). All culture mediums were supplemented with 10% (v/v) heat-inactivated fetal bovine serum and antibiotics. The cells were grown at 37°C with 5% CO₂.

The human colon adenocarcinoma cell lines, LS180 and Caco-2, were purchased from the European Collection of Cell Cultures (ECACC) (EC87021202-F0 and EC86010202-F0; KAC, Hyogo, Japan). LS180 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 1500 mg/L glucose (FUJIFILM Wako Pure Chemical) and Caco-2 cells were cultured in DMEM with 4500 mg/L glucose (FUJIFILM Wako Pure Chemical). In both the cases, the medium was supplemented with heat-inactivated 10% fetal bovine serum (BioWest, Nuaillé, France). The cultures were maintained at 37 °C in a humidified atmosphere with 5% CO₂.