The
B260300
Manufactured by BD
The B260300 is a laboratory equipment designed for centrifugation. It is a compact and versatile device that can separate different components of a liquid sample through the application of centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using b260300
1
Cell Culture Protocols for Drosophila and Tick Species
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D. melanogaster S2* cells were cultured with Schneider’s Drosophila medium (Gibco; 21720024) supplemented with 10% heat-inactivated FBS (Sigma; SH30070) and 1× Glutamax. Cells were maintained in T75 culture flasks (Corning; 353136) at 28°C.
TheI. scapularis tick cell line ISE6 was cultured at 32°C and 1% CO2 in L15C-300 medium supplemented with 10% heat-inactivated FBS (Sigma; F0926), 10% tryptose phosphate broth (TPB; BD; B260300) and 0.1% lipoprotein bovine cholesterol (LPBC; MP Biomedicals; 219147680) (35 (link)). The D. andersoni tick cell line, DAE100, was maintained at 34°C and cultured in L15B medium supplemented with 5% FBS, 10% TPB, and 1% LPBC as previously described (132 (link), 133 (link)).
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2
Cultivation of Tick and Human Cell Lines
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I. scapularis embryonic cell lines ISE6 and IDE12 were cultured at 32°C with 1% CO2 in L15C-300 and L15C media, respectively. These growth media were supplemented with 10% heat-inactivated FBS (Sigma, F0926), 10% tryptose phosphate broth (TBP; BD, B260300), and 0.1% lipoprotein bovine cholesterol (LPBC; MP Biomedicals, 219147680)20 (link),95 (link).
HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals; S11550) and 1x Glutamax. Cells were maintained in T75 culture flasks (Corning; 353136) at 33°C or 37°C in 5% CO2.
HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals; S11550) and 1x Glutamax. Cells were maintained in T75 culture flasks (Corning; 353136) at 33°C or 37°C in 5% CO2.
3
Culturing Tick and Human Cell Lines
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I. scapularis embryonic cell lines ISE6 and IDE12 were cultured at 32°C with 1% CO2 in L15C-300 and L15C media, respectively. These growth media were supplemented with 10% heat-inactivated FBS (Sigma, F0926), 10% tryptose phosphate broth (BD, B260300) and 0.1% lipoprotein bovine cholesterol (MP Biomedicals, 219147680) (20 (link), 105 (link)).
HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals, S11550) and 1× Glutamax. Cells were maintained in T75 culture flasks (Corning, 353136) at 33°C or 37°C in 5% CO2.
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I. scapularis embryonic cell lines ISE6 and IDE12 were cultured at 32°C with 1% CO2 in L15C-300 and L15C media, respectively. These growth media were supplemented with 10% heat-inactivated FBS (Sigma, F0926), 10% tryptose phosphate broth (BD, B260300) and 0.1% lipoprotein bovine cholesterol (MP Biomedicals, 219147680) (20 (link), 105 (link)).
HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals, S11550) and 1× Glutamax. Cells were maintained in T75 culture flasks (Corning, 353136) at 33°C or 37°C in 5% CO2.
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