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α pe magnetic microbeads

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α-PE magnetic microbeads are paramagnetic particles coated with anti-phycoerythrin (α-PE) antibody. They are designed for the isolation and enrichment of phycoerythrin-labeled cells from complex cell mixtures.

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Lab products found in correlation

Ls column, by Miltenyi Biotec (2 mentions) E50 2440, by BD (2 mentions)

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Magnetic microbeads (147 citations) α-PE microbeads (2 citations)

2 protocols using α pe magnetic microbeads

1

Eosinophil isolation and culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Eosinophils were isolated from the spleens of naïve Ly5.1+/+ mice by magnetic separation enrichment followed by flow cytometric sorting. Single cell suspensions of spleen were resuspended in MACS Buffer and then stained with an anti-Siglec-F (E50-2440, BD Biosciences, 1/100 dilution) antibody for 30 minutes on ice. Cell suspensions were washed and incubated with α-PE magnetic microbeads (Miltenyi Biotec) for 30 minutes on ice prior to enrichment of the Siglec-F-binding fraction using LS columns (Miltenyi Biotech). Live splenic eosinophils were then identified and purified as SSCAhiSiglec-F+Ly6G- cells. After washing cells with complete media, 5×104 purified eosinophils were cultured in complete media in 96 well plates supplemented with or without ILC2-conditioned media (ratio 1:1). After overnight culture, cells were harvested, washed twice in PBS before resuspension in RLT+ (RNA later + β-Mercaptoethanol) buffer and stored at -80°C until RNA extraction.
Jacquelot N., Seillet C., Wang M., Pizzolla A., Liao Y., Hediyeh-zadeh S., Grisaru-Tal S., Louis C., Huang Q., Schreuder J., Souza-Fonseca-Guimaraes F., de Graaf C.A., Thia K., Macdonald S., Camilleri M., Luong K., Zhang S., Chopin M., Molden-Hauer T., Nutt S.L., Umansky V., Ciric B., Groom J.R., Foster P.S., Hansbro P.M., McKenzie A.N., Gray D.H., Behren A., Cebon J., Vivier E., Wicks I.P., Trapani J.A., Munitz A., Davis M.J., Shi W., Neeson P.J, & Belz G.T. (2021). Blockade of the co-inhibitory molecule PD-1 unleashes ILC2-dependent anti-tumor immunity in melanoma. Nature immunology, 22(7), 851-864.
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2

Eosinophil isolation and culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Eosinophils were isolated from the spleens of naïve Ly5.1+/+ mice by magnetic separation enrichment followed by flow cytometric sorting. Single cell suspensions of spleen were resuspended in MACS Buffer and then stained with an anti-Siglec-F (E50-2440, BD Biosciences, 1/100 dilution) antibody for 30 minutes on ice. Cell suspensions were washed and incubated with α-PE magnetic microbeads (Miltenyi Biotec) for 30 minutes on ice prior to enrichment of the Siglec-F-binding fraction using LS columns (Miltenyi Biotech). Live splenic eosinophils were then identified and purified as SSCAhiSiglec-F+Ly6G- cells. After washing cells with complete media, 5×104 purified eosinophils were cultured in complete media in 96 well plates supplemented with or without ILC2-conditioned media (ratio 1:1). After overnight culture, cells were harvested, washed twice in PBS before resuspension in RLT+ (RNA later + β-Mercaptoethanol) buffer and stored at -80°C until RNA extraction.
Jacquelot N., Seillet C., Wang M., Pizzolla A., Liao Y., Hediyeh-zadeh S., Grisaru-Tal S., Louis C., Huang Q., Schreuder J., Souza-Fonseca-Guimaraes F., de Graaf C.A., Thia K., Macdonald S., Camilleri M., Luong K., Zhang S., Chopin M., Molden-Hauer T., Nutt S.L., Umansky V., Ciric B., Groom J.R., Foster P.S., Hansbro P.M., McKenzie A.N., Gray D.H., Behren A., Cebon J., Vivier E., Wicks I.P., Trapani J.A., Munitz A., Davis M.J., Shi W., Neeson P.J, & Belz G.T. (2021). Blockade of the co-inhibitory molecule PD-1 unleashes ILC2-dependent anti-tumor immunity in melanoma. Nature immunology, 22(7), 851-864.
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