RAW264.7 cells were treated with designated steroids at 100 nm 1 h before overnight LPS stimulation of inflammation. Each treatment was duplicated. The following day, RNA samples were isolated using a Qiagen mini RNA extraction kit and applied to the Agilent Whole Mouse Genome 8×60K gene expression platform. Data were analyzed by the limma package [45 (link)] in R program. Heat maps were generated by the HeatMap 2 package. Pathway analysis was done via DAVID Bioinformatics Resources 6.7 (NIAID) and Venn diagrams were generated by BioVenn [46 (link)].
Mini rna extraction kit
Manufactured by Agilent Technologies
The Mini RNA Extraction Kit is a laboratory equipment designed for the rapid and efficient extraction of RNA from a variety of sample types. It is a self-contained system that provides all the necessary reagents and components for the RNA extraction process.
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Lab products found in correlation
2 protocols using mini rna extraction kit
1
Steroids Modulate LPS-Induced Inflammation
2
Steroids Modulate LPS-induced Inflammation
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RAW264.7 cells were treated with designated steroids at 100 nM 1 h before overnight LPS stimulation of inflammation. Each treatment was duplicated. The following day, RNA samples were isolated using a Qiagen mini RNA extraction kit and applied to the Agilent Whole Mouse Genome 8x60K gene expression platform. Data were analyzed by the limma package [45 (link)] in R program. Heat maps were generated by the HeatMap 2 package. Pathway analysis was done via DAVID Bioinformatics Resources 6.7 (NIAID) and Venn diagrams were generated by BioVenn [46 (link)].
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