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Apoaf 50tst

Manufactured by Merck Group
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The APOAF-50TST is a laboratory equipment product manufactured by Merck Group. It is designed for a core laboratory function, but a detailed and unbiased description cannot be provided without the risk of extrapolation or interpretation. Therefore, a more comprehensive description is not available at this time.

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Lab products found in correlation

Fc500 flow cytometry, by Beckman Coulter (2 mentions) F10797, by Thermo Fisher Scientific (1 mentions) Cellquest software, by BD (1 mentions) Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions)

4 protocols using apoaf 50tst

1

TNF-α and DKK1 Impact on Apoptosis and Cell Cycle

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NP cells were stimulated with TNF‐α and DKK1, either alone or in combination with the transfection of miR‐640 mimics or a miR‐640 inhibitor, and then cultured at 37°C with 5% CO2 in humid air. The cells were washed twice with PBS and centrifuged. For apoptosis detection, discarded the supernatants and the cells were resuspended in 1 × annexin‐binding buffer. The apoptosis rate was detected by staining with Annexin V‐FITC and propidium iodide (Millipore, APOAF‐50TST). For cell cycle analysis, the cells were fixed in 70% ethanol overnight and underwent propidium iodide staining (Thermo, F10797). Finally, all samples were detected on FC500 flow cytometry (Beckman).
Dong W., Liu J., Lv Y., Wang F., Liu T., Sun S., Liao B., Shu Z, & Qian J. (2019). miR‐640 aggravates intervertebral disc degeneration via NF‐κB and WNT signalling pathway. Cell Proliferation, 52(5), e12664.
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2

Annexin V-FITC Apoptosis Assay in HEI-OC1 Cells

Cited 1 time
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HEI-OC1 cells were treated with or without H2O2 and NE at 37 °C. Then, the detailed procedures of flow cytometry analysis are described as previously [32 (link)]. For the measurement of apoptosis, discarded the supernatants and the cells were resuspended in 1 × annexin-binding buffer. The apoptosis rate was detected by staining with Annexin V-FITC and propidium iodide (Millipore, APOAF-50TST). Finally, all samples were detected on FC500 flow cytometry (Beckman).
Tian C., Yang Y., Wang R., Li Y., Sun F., Chen J, & Zha D. (2024). Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptor. BMC Neuroscience, 25, 5.
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3

Assessing hPASMC Proliferation and Apoptosis

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Proliferation of hPASMCs was assessed by flow cytometry with the CellTrace CFSE Cell Proliferation Kit (C34554, Molecular Probes) according to the manufacturer’s instructions. Analysis gates were set on live cells defined by scatter characteristics. The discrete peaks (proliferating smooth muscle cells) representing successive generations were gated for comparison to the undivided parent generation. For apoptosis assays, treated hPASMCs were processed with Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (APOAF-50TST, Sigma). Cells that were positive for Annexin V FITC staining and negative for propidium iodide staining were gated as the early apoptotic smooth muscle cells. Data were acquired by flow cytometry using CELLQuest software (Becton-Dickinson, Bedford, MA) and analyzed with FlowJo Software (FlowJo, Ashland, OR).
Lin Q., Fan C., Skinner J.T., Hunter E.N., Macdonald A.A., Illei P.B., Yamaji-Kegan K, & Johns R.A. (2019). RELMα Licenses Macrophages for DAMP Activation to Instigate Pulmonary Vascular Remodeling. Journal of immunology (Baltimore, Md. : 1950), 203(11), 2862-2871.
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4

Quantifying Apoptosis via TUNEL Assay

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Apoptotic cells were stained via the TUNEL technique using an apoptosis detection kit (cat. no. APOAF-50TST; Sigma-Aldrich; Merck KGaA) following the manufacturer's protocol. The sections were added onto a coverslip with DPX mounting medium (cat. no. 06522; Sigma-Aldrich; Merck KGaA). The total number of cells and the number of positive cells were counted in two sections from each animal (at magnification, ×400) in at least 10 fields of view in each section. The apoptosis index (AI) was calculated using the following formula: AI (%)=number of apoptotic cells/number of total cells ×100%.
Kong Q., Wu X., Duan W., Zhan L, & Song X. (2019). Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway. Experimental and Therapeutic Medicine, 17(5), 3598-3606.
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