Ferrous sulfate heptahydrate

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom, Germany, United States

Ferrous sulfate heptahydrate is a chemical compound with the formula FeSO4•7H2O. It is a crystalline solid that is used as a source of iron in various applications, including in the production of supplements, pharmaceuticals, and as a component in some industrial processes.

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7 protocols using ferrous sulfate heptahydrate

Synthesis and Characterization of Iron Oxide Nanoparticles

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Trimesic acid (BTC) (98% purity), ferrous sulfate heptahydrate (FeSO₄·7H₂O) (>98% purity), ferric nitrate nonahydrate (Fe(NO₃)₃·9H₂O) (>98% purity), sodium hydroxide (NaOH) (>97% purity), n-octane (≥99% purity), and ethanol (99.8% HPLC grade) were purchased from Fisher Scientific, UK with laboratory reagent grade. Nitric acid (ACS, Reag. 69%) was provided by EMSURE. Hydrogen gas of high purity (grade 4, 99.99%) was produced by the utilization of an on-demand hydrogen generation system known as H-Genie®. This process involved water electrolysis, resulting in the generation of hydrogen gas under pressures of up to 100 bar. A purification system is typically placed downstream of the electrolyzer to achieve a purity level of grade 5, with a minimum of 99.999% purity. All other gases of grade 5 (99.999% purity) are provided or imported by Air Supply Co., Egypt.

Ahmed H.E., Albolkany M.K., El-Khouly M.E, & El-Moneim A.A. (2024). Tailoring MIL-100(Fe)-derived catalyst for controlled carbon dioxide conversion and product selectivity. RSC Advances, 14(20), 13946-13956.

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Synthesis and Characterization of Immunomodulatory Nanocomposites

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Poly-l-lysine hydrobromide (PLL,
molecular weight: 30–70 kDa) and ammonium hydroxide solution
were purchased from Sigma-Aldrich. Ferric chloride hexahydrate (FeCl₃·6H₂O) and ferrous sulfate heptahydrate (FeSO₄·7H₂O) were purchased from Fisher Scientific.
Citric acid was purchased from Acros Organics. CpG oligodeoxynucleotides
1826 (sequence: T*C*C*A*T*G*A*C*G*T*T*C*C*T*G*A*C*G*T*T)
was customized from Integrated DNA Technologies (IDT). IL2 was obtained
from National Cancer Institute (NCI). α-CTLA-4 (anti-CTLA-4,
IgG2c, clone 9D9) was produced and purified by Neoclone. The information
on Taqman probes used for RT-qPCR is listed in Table S2. The information on antibodies used for flow cytometry
and immunohistochemistry is listed in Table S3.

Zhang Y., Rahman M.M., Clark P.A., Sriramaneni R.N., Havighurst T., Kerr C.P., Zhu M., Jones J., Wang X., Kim K., Gong S, & Morris Z.S. (2023). In Situ Vaccination Following Intratumoral Injection of IL2 and Poly-l-lysine/Iron Oxide/CpG Nanoparticles to a Radiated Tumor Site. ACS Nano, 17(11), 10236-10251.

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Synthesis and Characterization of Nanoparticles

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Poly-L-lysine hydrobromide (PLL) with a molecular weight of 30 to 70 kDa, ammonium hydroxide (NH₄OH) solution and sucrose were purchased from Sigma-Aldrich. Ferric chloride hexahydrate (FeCl₃·6H₂O) and ferrous sulfate heptahydrate (FeSO₄·7H₂O) were purchased from Fisher Scientific. Citric acid was obtained from Acros Organics. CpG oligodeoxynucleotides 1826 (CpG 1826) was purchased from Integrated DNA Technologies. Fluorescein isothiocyanate (FITC) was purchased from Chemodex Ltd. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from VWR International, LLC. Cyanine5-NHS easter (Cy5-NHS) was purchased from Lumiprobe. Ovalbumin (Ova) and fluorescein isothiocyanate labeled ovalbumin (FITC-Ova) were purchased from ThermoFisher. Macrophage colony-stimulating factor (M-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin 4 (IL-4) were purchased from Biolegend. The information of Taqman probes used for RT-qPCR and the information of antibodies used for flow cytometry and immunofluorescence staining are listed in Supplementary Table 4 and Supplementary Table 5 in the Supplementary Information, respectively. α-CTLA-4 (IgG2c, clone 9D9) was produced and purified by Neoclone.

Zhang Y., Sriramaneni R.N., Clark P.A., Jagodinsky J.C., Ye M., Jin W., Wang Y., Bates A., Kerr C.P., Le T., Allawi R., Wang X., Xie R., Havighurst T.C., Chakravarty I., Rakhmilevich A.L., O’Leary K.A., Schuler L.A., Sondel P.M., Kim K., Gong S, & Morris Z.S. (2022). Multifunctional nanoparticle potentiates the in situ vaccination effect of radiation therapy and enhances response to immune checkpoint blockade. Nature Communications, 13, 4948.

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Cellulose Powder Characterization and Applications

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Expired cellulose powder (chemically prepared ashless powder) originally obtained from W&R Balston, Ltd Genuine Whatman, England was supplied by the chemical store at the department of Chemistry in the American University in Cairo, Egypt. For comparison, fresh cellulose powder for column chromatography was purchased from Loba Chemie, pvt Ltd. Fluoxetine HCl (purity 100.3%) was cordially provided by Amoun Pharmaceuticals, Egypt. Methylene blue (NLT 85%) was purchased from Advent CHEMBIO Ltd™, India. Sodium hydroxide pellets (purity >97%) were obtained from Fisher, Germany, HCl (37% by vol.) of analytical grade was obtained from Acros, Germany, ethanol absolute (99.9%) was obtained from Fisher, Germany, and orthophosphoric acid (85%) and acetone (extra pure) were brought from Piochem, Egypt. The magnetic precursors anhydrous ferric chloride (97% purity) and ferrous sulfate heptahydrate (98% purity) were purchased from Fisher, Germany. NaCl (purity ≥99.5%) was obtained from Fisher Scientific, Loughborough, UK.

Farghal H.H., Nebsen M, & El-Sayed M.M. (2023). Exploitation of expired cellulose biopolymers as hydrochars for capturing emerging contaminants from water. RSC Advances, 13(29), 19757-19769.

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Reagents and Chemicals for Analytical Methods

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Aldehyde and 4-heptanone standards, bovine albumin serum, biuret reagent, HPLC grade methanol, water, formic acid, acetonitrile, ferrous sulfate heptahydrate and sodium phosphate dibasic anhydrous were purchased from Fisher Scientific (Tustin, CA, USA). Sodium phosphate monobasic was from Spectrum Chemical (Gardena, CA, USA). Ellman's reagent 5, 5'dithiobis(2-nitrobenzoic acid), EDTA, ferrozine , trichloroacetic acid (TCA), 2-Thiobarbituric acid (TBA), and 1, 1, 3, 3,-tetramethoxypropane (TMP) were purchased from Sigma-Aldrich (St.
Louis, MO, USA).

Jully K.M.M., Toto C.S, & Were L. (2016). Antioxidant effect of spent, ground, and lyophilized brew from roasted coffee in frozen cooked pork patties. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 66.

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Siderophore-Mediated Iron Homeostasis

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Ferrous sulfate heptahydrate and sodium dihydrogen citrate were obtained from Alfa Aesar (Ward Hill, MA). Dextran sodium sulfate (DSS) was obtained from MP Biomedicals, LLC (Solon, OH). Epigallocatechin-3-gallate, lipopolysaccharide (LPS; E. coli O128:B12), ferric chloride hexahydrate, pyrocatechol, deferoxamine mesylate salt, enterobactin (Escherichia coli), pyoverdines (Pseudomonas fluorescens), ferrichrome (Ustilago sphaerogena) were purchased from Sigma-Aldrich (St. Louis, MO). Salmonella typhimurium (SL3201) was a generous gift from Dr. Andrew Gewirtz (Georgia State University). All other fine chemicals used were reagent grade and procured from Sigma-Aldrich (St. Louis, MO).

Xiao X., Yeoh B.S., Saha P., Tian Y., Singh V., Patterson A.D, & Vijay-Kumar M. (2016). Modulation of Urinary Siderophores by the Diet, Gut Microbiota and Inflammation in Mice. The Journal of nutritional biochemistry, 41, 25-33.

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Inflammatory Marker Quantification in Mouse

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Duoset mouse MPO, neutrophil elastase (ELA2), lipocalin 2 (Lcn2), serum amyloid A (SAA), interleukin (IL)-6, IL-1β, and CXCL1 (KC) ELISA kits were obtained from R&D Systems. Aspartate transaminase (AST) and alanine transaminase (ALT) kits were purchased from Randox Laboratories. Ferrous sulfate heptahydrate was obtained from Alfa Aesar. 4-aminobenzoic acid hydrazide (ABAH) was procured from Sigma. qScript cDNA synthesis kit and SYBR® Green mix was acquired from Quanta Biosciences. All other fine chemicals used in present study were reagent grade and procured from Sigma.

Xiao X., Saha P., Yeoh B.S., Hipp J.A., Singh V, & Vijay-Kumar M. (2018). Myeloperoxidase Deficiency Attenuates Systemic and Dietary Iron-induced Adverse Effects. The Journal of nutritional biochemistry, 62, 28-34.

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