Accustain crystal violet solution

Manufactured by Merck Group

Accustain Crystal Violet solution is a laboratory reagent used for cell and tissue staining. It contains a dye called crystal violet, which binds to cellular components and produces a purple color. The solution is designed for use in various histological and cytological applications, such as microscopic examination of cells and tissues.

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Crystal violet (5440 citations) Crystal violet solution (775 citations) Accustain (22 citations)

2 protocols using accustain crystal violet solution

Colony Formation Assay Protocol

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For colony formation assays, cells were plated in triplicate (500 cells/well) in 6-well plates or 100 cells/well in 24-well plates and grown overnight before compounds were added. After 1– 2 weeks, depending on cell line, colonies were stained with Accustain Crystal Violet solution (Sigma-Aldrich) for 30 min. Plates were rinsed with water and images were acquired by scanning. Colony number and size were analyzed using Image J software. Colony formation efficiency was calculated relative to the number of colonies in control (DMSO)-treated wells.

Feng Y., Sessions E.H., Zhang F., Ban F., Placencio-Hickok V., Ma C.T., Zeng F.Y., Pass I., Terry D.B., Cadwell G., Bankston L.A., Liddington R.C., Chung T.D., Pinkerton A.B., Sergienko E., Gleave M., Bhowmick N., Jackson M.R., Cherkasov A, & Ronai Z.A. (2019). Identification and characterization of small molecule inhibitors of the ubiquitin ligases Siah1/2 in melanoma and prostate cancer cells. Cancer letters, 449, 145-162.

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Colony Formation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

For colony formation assays, cells were plated in triplicate (500 cells/well) in 6-well plates or 100 cells/well in 24-well plates and grown overnight before compounds were added. After 1–2 weeks, depending on cell line, colonies were stained with Accustain Crystal Violet solution (Sigma-Aldrich) for 30 min. Plates were rinsed with water and images were acquired by scanning. Colony formation efficiency was calculated relative to the number of colonies in control (DMSO)-treated wells.

Feng Y., Pathria G., Heynen-Genel S., Jackson M., James B., Yin J., Scott D.A, & Ronai Z.A. (2021). Identification and Characterization of IMD-0354 as a Glutamine Carrier Protein Inhibitor in Melanoma. Molecular cancer therapeutics, 20(5), 816-832.

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