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Chmp4c is a protein that plays a role in the regulation of cell division and cell death. It is involved in the formation of the contractile ring during cytokinesis, the final stage of cell division. The protein belongs to the Charged multivesicular body protein 4 (CHMP4) family, which are components of the ESCRT-III complex that facilitates the separation of daughter cells during cell division.

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2 protocols using chmp4c

1

siRNA Knockdown of CHMP4 Proteins

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Negative siRNAs (a pool of four different siRNAs: 5′-UAAGGCUAUGAAGAGAUAC-3′, 5′-AUGUAUUGGCCUGUAUUAG-3′, 5′-AUGAACGUGAAUUGCUCAA-3′, and 5′-UGGUUUACAUGUCGACUAA-3′) were from Thermo Scientific. Human Chmp4c located in the open reading frame (5′-GCUUGGGCUACCUAAACUA-3′), Chmp4c-2 located in the 3′-untranslated region (5′-GUAGAGGAGUCUUAUAUGA-3′), ZW10 (a pool of three different siRNAs: 5′-GCAUGGAGCUCACAAUACA-3′, 5′-CCAAGUAUUUGCACCUUUA-3′, and 5′-CAAGCCAGCUUGCAAGAAA-3′), Chmp4a (a pool of three different siRNAs: 5′-CAAACUGACGGGACAUUAU-3′, 5′-CUGAGUGGGUAUCCUGAUA-3′, and 5′-GAAGGAUCUUGCUACAGAA-3′), and Chmp4b (a pool of three different siRNAs: 5′-CAACAUGGACAUCGAUAAA-3′, 5′-CAGUCCCUCUACCAAAUGU-3′, and 5′-GCUGAAUCCUCAAUGGAAA-3′) siRNAs were obtained from Santa Cruz Biotechnology. Only the sense sequences of the siRNA duplexes are shown.
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2

Investigating Cellular Signaling Pathways

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Negative siRNA (a pool of four different siRNAs: 5′-UAAGGCUAUGAAGAGAUAC-3′, 5′-AUGUAUUGGCCUGUAUUAG-3′, 5′-AUGAACGUGAAUUGCUCAA-3′, 5′-UGGUUUACAUGUCGACUAA-3′) and human Aurora B siRNA (5′-CCAAACUGCUCAGGCAUAA-3′) were from Thermo Scientific. Human Clk1 (5′-GUAAACCUCUGAAGGAAUU-3′), Clk2 (5′-CCUUCGAUUUCCUCAAAGA-3′), Clk4 (5′-GCAAACCGUUGAAGGAAUU-3′), Clk1b (5′-GUAGACAUGUAGCAGUAAA-3′), Clk2b (5′-CAGCUCGACUUGAGAUCAA-3′), Clk4b (5′-GGAAAGGCAUGCAGUUUGU-3′), Chmp4c (a pool of three different siRNAs: 5′-GCUUGGGCUACCUAAACUA-3′, 5′-GUCAUGUGCAUACAUAGAA-3′, 5′-GUAGAGGAGUCUUAUAUGA-3′), CENP-A (a pool of three different siRNAs: 5′-GCAUGACUUUCCUCUGUAA-3′, 5′-CUAGUAAAUUCCUGUCAAA-3′, 5′-GUAUCAUAACAGUUCAGAA-3′) and Nup153 (5′-AAGGCAGACUCUACCAAAUGUUU-3′) siRNAs were from Santa Cruz Biotechnology. Only the sense sequences of the siRNA duplexes are shown and all sequences are provided in 5′→3′ orientation.
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