Sh uca1

Human GBC cell lines (SGC-996, GBC-SD, and NOZ) and human intrahepatic bile duct epithelial cells (HIBEpiC) were used in the study. SGC-996, GBC-SD, and HIBEpiC cells were obtained from Jingkang Biological Engineering Co., Ltd. (Shanghai, China). NOZ cells were obtained from Bena culture collection (Suzhou, China). Roswell Park Memorial Institute (RPMI) 1640 medium (Thermo Fisher Scienti c, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scienti c) and 100 U/mL penicillin/streptomycin (Corning Inc., Corning, NY, USA) was used to culture all cell lines. All cell lines were incubated in an atmosphere with 5% CO 2 at 37˚C. Short hairpin RNA (sh-RNA) targeting UCA1 (sh-UCA1) and negative control (sh-NC) were synthesized by Genepharma (Shanghai, China). The sequences of UCA1 and SPOCK1 were cloned into the pcDNA3.1 vector (vector) (Invitrogen, Carlsbad, CA, USA) to construct the overexpression vectors for UCA1 and SPOCK1, respectively. MiR-613 mimic and inhibitor (miR-613 and anti-miR-613) and their corresponding negative controls (miR-NC and anti-miR-NC) were purchased from Genepharma. Oligonucleotides or plasmids were transfected into NOZ and GBC-SD cells using the lipofectamine 2000 reagent (Thermo Fisher Scienti c).