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Sypro orange protein stain dye

Manufactured by Merck Group
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SYPRO Orange protein stain dye is a fluorescent dye used for the detection and quantitation of proteins in various laboratory applications. It binds non-covalently to proteins and emits a bright orange fluorescence upon excitation, allowing for sensitive and specific protein visualization.

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Lab products found in correlation

Opticon monitor 3.1 analysis software, by Bio-Rad (1 mentions)

Close spelling variants of this product

Sypro Orange protein stain SYPRO Orange dye SYPRO Orange

2 protocols using sypro orange protein stain dye

1

Protein Thermal Stability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Assay mixtures of 50 μl containing 0.6 mg/ml protein, 20 mM Bis-Tris propane, pH 7.0 and SYPRO Orange protein stain dye (Sigma). Melting curves were recorded with a temperature range of 20 to 95 °C, using a DNA Engine Opticon 3 real time PCR system (MJ Research) with Opticon Monitor 3.1 analysis software (BioRad).
Syson K., Batey S.F., Schindler S., Kalscheuer R, & Bornemann S. (2021). A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and the accumulation of α-maltose-1-phosphate. Biochimica et Biophysica Acta. General Subjects, 1865(2), 129783.
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2

Thermal Stability Assay of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Assay mixtures of 50 µl containing 0.6 mg/ml protein, 20 mM Bis-Tris propane, pH 7.0 and SYPRO Orange protein stain dye (Sigma). Melting curves were recorded with a temperature range of 20 to 95 °C, using a DNA Engine Opticon 3 real time PCR system (MJ Research)
with Opticon Monitor 3.1 analysis software (BioRad).
Syson K., Batey S.F.D., Schindler S., Kalscheuer R., & Bornemann S. (2020). A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and the accumulation of α-maltose-1-phosphate.
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